VP7 and VP4 genotypes among rotavirus strains recovered from children with gastroenteritis over a 3-year period in Valencia, Spain
Cite this article as: Buesa, J., de Souza, C., Asensi, M. et al. Eur J Epidemiol (2000) 16: 501. doi:10.1023/A:1007618215377 Abstract
Between September 1996 and May 1999, the incidence and distribution of the main human rotavirus G genotypes (VP7 associated: G1–G4) and P genotypes (VP4 associated: P, P, P and P) among children with rotavirus gastroenteritis were determined using reverse transcription and polymerase chain reaction (RT-PCR)-based genotyping methods. From a total of 145 rotavirus strains examined, we identified the G type in 131 (90.3%) and the P type in 127 (87.5%) of the samples. An overall predominance of genotypes P G1 (42.7%) and P G4 (32.4%) was found during the period of study, with much lower incidence of genotypes P G2 (5.5%) and P G3 (2%). P and P types were not detected, neither were unusual combinations of P and G types. A significant genotypic shift was observed: whereas P G4 was the most prevalent genotype during the first year of the study (60%), the genotype P G1 gradually increased to account for 62.3% of the strains analysed in the following winter season. Mixed G types revealing dual infections G1/G4 and G3/G4 were found at low frequency (2%).
Genotypes Reverse transcription and polymerase chain reaction (RT-PCR) Rotavirus VP4 VP7 References
Kapikian AZ, Chanock RM. Rotaviruses. In: Fields BN, Knipe DN, Howley PM, et al. (eds), Fields virology. Vol 2. Philadelphia: Lippincott-Raven, 1996: 1657–1708.
Midthun K, Kapikian AK. Rotavirus vaccines: An overview. Clin Microbiol Rev 1996; 9: 423–434.
CDC. Withdrawal of rotavirus vaccine recommendation. MMWR Morb Mortal Wkly Rep 1999; 48: 1007.
Gouvea V, Glass RI, Woods P, et al. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 1990; 28: 276–282.
Gentsch JR, Glass RI, Woods P, et al. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 1992; 30: 1365–1373.
Hoshino Y, Kapikian AZ. Classification of rotavirus VP4 and VP7 serotypes. Arch Virol 1996; 12: 99–111.
Gentsch JR, Woods PA, Ramachandran M, et al. Review of G and P typing results from a global collection of rotavirus strains: Implications for vaccine development. J Infect Dis 1996; 174(Suppl. 1): S30–S36.
Bishop RF, Unicomb LE, Barnes GL. Epidemiology of rotavirus serotypes in Melbourne, Australia, from 1973 to 1989. J Clin Microbiol 1991; 29: 862–868.
Noel JS, Beards GM, Cubitt WD. Epidemiological survey of human rotavirus serotypes and electropherotypes in young children admitted to two children's hospitals in northeast London from 1984 to 1990. J Clin Microbiol 1991; 29: 2213–2219.
Maunula L, van Bonsdorff CH. Rotavirus serotypes and electropherotypes in Finland from 1986 to 1990. Arch Virol 1995; 140: 877–890.
Leite JP, Alfieri AA, Woods P, Glass RI, Gentsch JR. Rotavirus G and P types circulating in Brazil: Characterization by RT-PCR, probe hybridization, and sequence analysis. Arch Virol 1996; 141: 2365–2374.
Cunliffe NA, Gondwe JS, Broadhead RL, et al. Rota-virus G and P types in children with acute diarrhea in Blantyre, Malawi, from 1997 to 1998: Predominance of novel PG8 strains. J Med Virol 1999; 57: 308–312.
Ramachandran M, Das BK, Vij A, et al. Unusual diversity of human rotavirus G and P genotypes in India. J Clin Microbiol 1996; 34: 436–439.
Ramachandran M, Gentsch JR, Parashar UD, et al. and the National Rotavirus Strain Surveillance System Collaborating Laboratories. Detection and characterization of novel rotavirus strains in the United States. J Clin Microbiol 1998; 36: 3223–3229.
Estes MK. Rotaviruses and their replication. In: Fields BN, Knipe DN, Howley PM, et al. (eds), Fields virology. Vol 2. Philadelphia: Lippincott-Raven, 1996: 1625–1655.
Buesa J, Colomina J, Raga J, Villanueva A, Prat J. Evaluation of reverse transcription and polymerase chain reaction (RT/PCR) for the detection of rotaviruses: Applications of the assay. Res Virol 1996; 147: 353–361.
Buesa FJ, Duato M, Gimeno C, García de Lomas J. Sequential variation in genomic RNA patterns of human rotaviruses isolated from infantile gastroenteritis. Ann Inst Pasteur/Virol 1987; 13: 307–314.
Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD. Rapid diagnosis of rotavirus infection by direct detection of nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 1982; 16: 473–477.
O'Mahony J, Foley B, Morgan S, Morgan JG, Hill C. VP4 and VP7 genotyping of rotavirus samples recovered from infected children in Ireland over a 3–year period. J Clin Microbiol 1999; 37: 1699–1703.
Visser LE, Cano Portero R, Gay NJ, Martínez Navarro JF. Impact of rotavirus disease in Spain: An esti-mate of hospital admissions due to rotavirus. Acta Paediatr 1999; 426(suppl): 72–77.
Buesa J, García-VerdÚ R, Ventura L. Subgrupos, serotipos y patrones electroforé ticos de ARN de rotavirus detectados durante 4 años en Valencia. Proceedings of the IV Congreso Nacional de la Sociedad Española de Microbiología y Enfermedades Infecciosas (SEI-MC), Madrid, Spain, 1990, abstract E2/2, p 350.
Cilla G, Gil A, López-Lopategui C, Iturzaeta A, Gutiérrez Z. Serotipos de rotavirus en Gipuzkoa: Análisis de 7 años. Proceedings of the VII Congreso Nacional de la Sociedad Española de Microbiologí a y Enfermedades Infecciosas (SEIMC), Torremolinos (Má laga), Spain, 1996, abstract 5/7, p 36.
Koopmans M, Brown D. Seasonality and diversity of group A rotaviruses in Europe. Acta Paediatr 1999; 426(suppl): 14–19.
Arista S, Vizzi E, Ferraro D, Cascio A, Di Stefano R. Distribution of VP7 serotypes and VP4 genotypes among rotavirus strains recovered from Italian children with diarrhea. Arch Virol 1997; 142: 2065–2071.
Gault E, Chicki-Brachet R, Delon S, et al. Distribution of human rotavirus G types circulating in Paris, France, during the 1997–1998 epidemic: High prevalence of type G4. J Clin Microbiol 1999; 37: 2373–2375.
Unicomb LE, Podder G, Gentsch JR, et al. Evidence of high-frequency genomic reassortment of group A rotavirus strains in Bangladesh: Emergence of type G9 in 1995. J Clin Microbiol 1999; 37: 1885–1891.
Iturriza-Gómara M, Green J, Brown DWG, Desselberger U, Gray JJ. Diversity within the VP4 gene of rotavirus P strains: Implications for reverse transcription-PCR genotyping. J Clin Microbiol 2000; 38: 898–901.
Adah MI, Rohwedder A, Olaleyle OD, Werchau H. Nigerian rotavirus serotype G8 could not be typed 505 by PCR due to nucleotide mutation at the 3¢end of the primer binding site. Arch Virol 1997; 142: 1881–1887.
Masendycz PJ, Palombo EA, Gorrell RJ, Bishop R. Comparison of enzyme immunoassay, PCR, and type-specific cDNA probe techniques for identification of group A rotavirus gene 4 types (P types). J Clin Microbiol 1997; 35: 3104–3108.
Iturriza-Gómara M, Green J, Brown DWG, Desselberger U, Gray JJ. Comparison of specific and random priming in the reverse-transcriptase polymerase chain reaction for genotyping group A rotavirus. J Virol Methods 1999; 78: 93–103.
Sanders RC. Molecular epidemiology of human rota-virus infections. Eur J Epidemiol 1985; 1: 19–32.
Zao C-L, Yu W-N, Kao C-L, Taniguchi K, Lee C-Y, Lee CN. Sequence analysis of VP1 and VP7 genes suggests occurrence of a reassortant of G2 rotavirus responsible for an epidemic of gastroenteritis. J Gen Virol 1999; 80: 1407–1415.
Clarke IN, McCrae MA. Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates. Infect Immun 1982; 36: 492–497.
Husain M, Seth P, Dar L, Broor S. Classification of rotavirus into G and P types with specimens from children with acute diarrhea in New Delhi, India. J Clin Microbiol 1996; 34: 1592–1594.
Google Scholar Copyright information
© Kluwer Academic Publishers 2000