Detection and typing of human papillomavirus DNA in paired urine and cervical scrapes
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
The prevalence of human papillomavirus (HPV) in paired cervical scrape and urine specimens from 144 women attending a clinic for genitourinary medicine was determined by polymerase chain reaction (PCR) and nested PCR, using degenerate and general primer pairs localized within the L1 region. HPV typing was by restriction fragment length polymorphism (RFLP), type-specific PCR (HPV 6, 11, 16, 18, 33), and partial DNA sequencing of PCR products. HPV DNA was detected in 114 (84%) women. HPV DNA was detected in the specimens of 58 patients after amplification with MY09/MY11 primers and in a further 54 patients after nested PCR with the GP5 + /GP6 + primers. A total of 106/136 (78%) of women had HPV DNA positive cervical scrapes and 89 (65%) had HPV DNA positive urine specimens. Both the urine and cervical specimens of 81 women were positive. In 25 women HPV DNA was detected in the cervical specimen only, and in 8 women HPV DNA was detected in the urine specimens only. A total of 108 specimens from 75 patients were typed. For 33 patients HPV typing was achieved in both the cervical and the urine specimens and 19 women had identical types in paired specimens. Multiple HPV infections could be detected in 15 (20%) of 75 women where either the cervical and urine specimen or both of the specimens could be typed. More then one HPV type was found in 8 specimens and from multiple sites (cervix and urinary tract) in the same patients on 7 occasions. The results of this study indicate that the detection of HPVs in the urogenital tract can be maximised through the testing of both cervical scrapes and urine specimens in conjunction with the use of a nested PCR to increase the sensitivity of HPV DNA detection. Also, urine cannot be a direct substitute for a cervical scrape as different HPV types are often detected in the urine compared with those detected in the cervix.
- Roman A, Fife KH. Human papillomaviruses: Are we ready to type? Clin Microbiol Reviews 1989; 2: 166–174.
- zur Hausen H. Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers. Cancer Res 1989; 49: 4677–4681.
- zur Hausen H. Human papillomavirus in the pathogenesis of anogenital cancer. Virology 1991; 184: 9–13.
- Munoz N, Bosch FX, de Sanjose S, Tafur L, Izarzugaza I, Gili M, Viladiu P, Navarro C, Martos C, Ascunce N, Gonzalez LC, Kaldor JM, Guerrero E, Lorincz A, Santamaria M, Alonso de Ruiz P, Aristizabal N, Shah K. The causal link between human papillomavirus and invasive cervical cancer: A population-based case-control study in Columbia and Spain. Int J Cancer 1992; 52: 743–749.
- Matsukura T, Sugase M. Molecular cloning of a novel human papillomavirus (type 58) from an invasive cervical carcinoma. Virology 1990; 177: 833–836.
- de Villiers E-M. Human pathogenic papillomavirus types: an update. Curr Top Microbiol Immunol 1994; 186: 1–12.
- Lorincz AT, Reid R, Jenson AB, Greenberg MD, Lancaster W, Kurman RJ. Human papillomavirus infection of the cervix: Relative risk associations of 15 common anogenital types. Obstet Gynecol 1992; 79: 328–337.
- Manos MM, Ting Y, Wright DK, Lewis AJ, Broker TR, Wolinsky SM. Use of PCR amplification for the detection of genital HPV. Cancer Cells 1989; 7: 209–214.
- Snijders PJF, van den Brule AJC, Schrijnemakers HJF, Snow G, Meijer CJLM, Walboomers JMM. The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of HPV genotypes. J Gen Virol 1990; 71: 173–181.
- Van den Brule AJC, Meijer CJLM, Bakels V, Kenemans P, Walboomers JMM. Rapid detection of human papillomavirus in cervical scrapes by combined general primer-mediated and type-specific polymerase chain reaction. J Clin Microbiol 1990; 28: 2739–2743.
- Melkert PWJ, Hopman E, van den Brule AJC, Risse EKJ, van Diest PJ, Bleker OP, Helmerhorst T, Schipper MEI, Meijer CJLM, Walboomers JMM. Prevalence of HPV in cytomorphologically normal cervical scrapes, as determined by the polymerase chain reaction, is age-dependent. Int J Cancer 1993; 53: 919–923.
- Eluf-Neto J, Booth M, Smith P, Munoz N, Bosch FX, Meijer CJLM, Walboomers JMM. Human papillomavirus and invasive cervical cancer in Brazil. Brit J Cancer 1994; 69: 114–119.
- Evander M, Edlund K, Boden E, Gustafsson A, Jonsson M, Karlson R, Rylander E, Wadell G. Comparison of a one-step and two-step polymerase chain reaction with degenerate general primers in a population-based study of human papillomavirus infection in young Swedish women. J Clin Microbiol 1992; 30: 987–992.
- Snijders PJ, de Roda Husman A-M, Walboomers JMM, van den Brule AJC, Meijer CJLM. The use of general primers GP5 and GP6 elongated at their 3′ ends with adjacent highly conserved sequences improves HPV detection by PCR. J Gen Virol 1995; 76: 1057–1062.
- Melchers WJG, Schiff R, Stolz E, Linderman J, Quint WGV. Human papillomavirus detection in urine samples from male patients by the polymerase chain reaction. J Clin Microbiol 1989; 27: 1711–1714.
- Forslund O, Hansson BG, Rymark P, Bjerre B. Human papillomavirus DNA in urine samples compared to that in simultaneously collected urethra and cervix samples. J Clin Microbiol 1993; 31: 1975–1979.
- Vossler JL, Forbes BA, Adelson MD. Evaluation of the polymerase chain reaction for the detection of human papillomavirus from urine. J Med Virol 1995; 45: 354–360.
- Boom R, Sol CJA, Salimaus MMM, Jansen CL, Dillen PME W-V, Noordaa JVD. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 28: 495–503.
- Maniatis T, Fritsch EF, Sambrook J. Molecular cloning. In: Dieffenbacher CW, Dvesksler GS (eds), A laboratory manual. Cold Spring Harbor Laboratory, New York: Cold Spring Harbor Laboratory Press, 1989; 150–162.
- Vahey MT, Wong MT, Michael NL. A standard PCR protocol: Rapid isolation of DNA and PCR assay for β-globin. In: Dieffenbacher CW, Dvesksler GS (eds), PCR Primer; Laboratory manual. Cold Spring Harbor Laboratory, New York: Cold Spring Harbor Laboratory Press, 1995: 17–29.
- Lungu O, Wright Jr TC, Silverstein S. Typing of human papillomavirus by polymerase chain reaction amplification with L1 consensus primers and RFLP analysis. Mol and Cell Probes 1991; 6: 145–152.
- Van den Brule AJC, Claas ECJ, du Maine M, Melchers WJG, Helmerhorst T, Quint WGV, Lindeman J, Meijer CJLM, Walboomers JMM. Use of anticontamination primers in the polymerase chain reaction for the detection of human papilloma virus genotypes in cervical scrapes and biopsies. J Med Virol 1989; 29: 20–27.
- Syrjanen S, Saastamoinen J, Chang F, Ji H, Syrjanen K. Colposcopy, punch biopsy, in situ DNA hybridization, and the polymerase chain reaction in searching for genital human papillomavirus (HPV) infections in women with normal PAP smears. J Med Virol 1990; 31: 259–266.
- Maki H, Saito S, Ibaraki T, Ichijo M, Yoshie O. Use of universial and type-specific primers in the polymerase chain reaction for the detection of genital human papillomaviruses. Jap J Cancer Res 1991; 82: 411–419.
- Sun XW, Kuhn L, Ellerbrock TV, Chiasson MA, Bush TJ, Wright TC. Human papillomavirus infection in women infected with the human immunodeficiency virus. N Engl J Med 1997; 337: 1343–1349.
- Tenti P, Zappatore R, Romagnoli S, Civard E, Giunta P, Scelsi R, Stella G, Carnevali L. p. 53 Overexpression and human papillomavirus infection in transitional cell carcinoma of the urinary bladder: Correlation with histological parameters. J Path 1996; 178: 65–70.
- Detection and typing of human papillomavirus DNA in paired urine and cervical scrapes
European Journal of Epidemiology
Volume 15, Issue 6 , pp 537-543
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- Cervical specimens
- DNA sequencing
- Human papillomavirus
- polymerase chain reaction (PCR)
- restriction fragment length polymorphism (RFLP)
- Urine specimens
- Industry Sectors
- Author Affiliations
- 1. Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, UK
- 2. Department of Molecular Microbiology, University of Southampton, Southampton General Hospital, Southampton, UK
- 3. Department of Genitourinary Medicine, Addenbrooke's Hospital, Cambridge, UK