Neurochemical Research

, Volume 25, Issue 5, pp 591–602

Searching for Depolarization-Induced Genes that Modulate Synaptic Plasticity and Neurotrophin-Induced Genes that Mediate Neuronal Differentiation

Authors

  • Harvey R. Herschman
    • Departments of Biological Chemistry. Pharmacology. Molecular Biology. Brain Research InstitutesUCLA
  • Gregory D. Ferguson
    • Departments of Biological Chemistry. Molecular Biology. Brain Research InstitutesUCLA
  • Jonathan D. Feldman
    • Pediatrics. Brain Research InstitutesUCLA
  • Robin Farias-Eisner
    • Obstetrics and Gynecology. Molecular Biology
  • Linda Vician
    • Departments of Biological Chemistry. Molecular Biology. Brain Research InstitutesUCLA
Article

DOI: 10.1023/A:1007546600535

Cite this article as:
Herschman, H.R., Ferguson, G.D., Feldman, J.D. et al. Neurochem Res (2000) 25: 591. doi:10.1023/A:1007546600535

Abstract

We identify and characterize two classes of immediate-early genes: (i) genes, induced by depolarization in neurons, that play a role in depolarization-induced neuronal plasticity and (ii) genes, induced in neuronal precursors by neurotrophins, that play a causal role in neurotrophin-directed neuronal differentiation. We use rat PC12 pheochromocytoma cells to identify (i) genes preferentially induced by [depolarization or forskolin] versus [Nerve Growth Factor (NGF) or Epidermal Growth Factor (EGF)] and (ii) genes preferentially induced by NGF versus EGF. We describe (i) a collection of genes preferentially induced by depolarization/forskolin in PC12 cells and by kainic acid in vivo, and (ii) a collection of genes preferentially induced by NGF. The synaptotagmin IV gene encodes a synaptic vesicle protein whose level is modulated by depolarization. NGF preferentially induces the urokinase-plasminogen activator receptor in PC12 cells. Antisense oligonucleotide and anti-UPAR antibody experiments demonstrate that NGF-induced UPAR expression is required for NGF-driven PC12 cell differentiation.

Depolarizationforskolinnerve growth factorsynaptotagminUPARPC12 cells

Copyright information

© Plenum Publishing Corporation 2000