Plant Molecular Biology Reporter

, Volume 16, Issue 2, pp 183–183

A Method for Isolation of Chloroplast DNA and Mitochondrial DNA from Sunflower

  • S.O. Triboush
  • N.G. Danilenko
  • O.G. Davydenko
Article

DOI: 10.1023/A:1007487806583

Cite this article as:
Triboush, S., Danilenko, N. & Davydenko, O. Plant Molecular Biology Reporter (1998) 16: 183. doi:10.1023/A:1007487806583
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Abstract

We present a method for isolation of chloroplast and mitochondrial DNA from sunflower seedlings. The protocol includes: organelle isolation, deoxyribonuclease treatment, lysis, deproteinisation and a final DNA purification with sodium dodecyl sulphate and potassium acetate. The organelle DNA yield is 5–10 micrograms per gram of tissue and the DNA is fully restrictable. The technique is inexpensive and appropriate for the isolation of multiple samples of organelle DNA from a small amount of tissue.

chloroplast DNA mitochondrial DNA organelle isolation sunflower 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • S.O. Triboush
    • 1
  • N.G. Danilenko
    • 1
  • O.G. Davydenko
    • 1
  1. 1.Department of Extranuclear Heredity, National Academy of SciencesInstitute of Genetics & CytologyMinskBelarus

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