, Volume 16, Issue 2, p 133

Isolation of DNA from the Highly Mucilagenous Succulent Plant shape Sedum telephium

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The C3-CAM intermediate plant Sedum telephium is very rich in mucilages (gelling polysaccharides) which present a major obstacle to the isolation of DNA. We have developed an extraction procedure that involves a step-wise increase in the concentration of CTAB (cetyl-trimethylammonium bromide) finally yielding a nucleic acid precipitate that is free from polysaccharides. DNA may be further purified by differential precipitation with LiCl and/or RNase digestion. The DNA has excellent spectral qualities, is readily accessible to restriction endonucleases and is usable as a PCR template.