Isolation of DNA from the Highly Mucilagenous Succulent Plant shape Sedum telephium
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- Barnwell, P., Blanchard, A.N., Bryant, J.a. et al. Plant Molecular Biology Reporter (1998) 16: 133. doi:10.1023/A:1007473302551
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The C3-CAM intermediate plant Sedum telephium is very rich in mucilages (gelling polysaccharides) which present a major obstacle to the isolation of DNA. We have developed an extraction procedure that involves a step-wise increase in the concentration of CTAB (cetyl-trimethylammonium bromide) finally yielding a nucleic acid precipitate that is free from polysaccharides. DNA may be further purified by differential precipitation with LiCl and/or RNase digestion. The DNA has excellent spectral qualities, is readily accessible to restriction endonucleases and is usable as a PCR template.