Plant Molecular Biology Reporter

, Volume 16, Issue 2, pp 175–175

Efficient Amplification of Insert End Sequences from Bacterial Artificial Chromosome Clones by Thermal Asymmetric Interlaced PCR


  • Yao-Guang Liu
    • Biotechnology CenterSouth China Agricultural University
  • Ning Huang
    • International Rice Research Institute

DOI: 10.1023/A:1007420918645

Cite this article as:
Liu, Y. & Huang, N. Plant Molecular Biology Reporter (1998) 16: 175. doi:10.1023/A:1007420918645


TAIL-PCR is a powerful tool for the recovery of DNA fragments adjacent to known sequences. A protocol is presented for the amplification of insert end sequences from bacterial artificial chromosome clones using TAIL-PCR. The amplified products are suitable as probes for chromosome walking and genome mapping and as templates for direct sequencing. The protocol has been used in rice genome studies. Abbreviations: AD primer, arbitrary degenerate primer; BAC, bacterial artificial chromosome.

bacterial artificial chromosomechromosome walkinginsert end sequences

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© Kluwer Academic Publishers 1998