Plant Molecular Biology Reporter

, Volume 16, Issue 1, pp 91–91

A Simple and Rapid Procedure for Isolation of Total DNA Suitable for Fingerprint Analysis from shape Amaranthus

  • Anupam Dixit
Article

DOI: 10.1023/A:1007414505189

Cite this article as:
Dixit, A. Plant Molecular Biology Reporter (1998) 16: 91. doi:10.1023/A:1007414505189

Abstract

A simple, efficient and reliable method is described for isolation of total DNA from young leaves of Amaranthus species. This procedure yields a high amount (600–800 µg DNA/g fresh leaf tissue) of good quality DNA free from contaminating proteins, polysaccharides, and coloured pigments. The DNA is suitable for digestion with several restriction endonucleases, preparation of Southern blots, and PCR amplification. The DNA has been successfully used for generating DNA fingerprint profiles and RAPD banding patterns in two species of Amaranthus. The procedure is suitable for processing of a large number of samples simultaneously.

Amaranthus DNA fingerprinting PCR polysaccharides RAPD total DNA 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Anupam Dixit
    • 1
  1. 1.Centre for Plant Molecular BiologyNational Botanical Research InstituteLucknowIndia