Detection of BK polyomavirus genotypes in healthy and HIV-positive children
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Urine samples from 211 community children (3–7 years age), from 33 HIV type-1 infected children and from 56 HIV- negative children were collected and analyzed for the presence of BK virus (BKV) DNA by PCR. PCR amplifications were carried out using primers specific for the BKV structural region VP1. We also investigated the distribution of BKV subtypes by a restriction fragment polymorphism assay (RFLP). We demonstrated BKV DNA in 3.8% of 211 community children with a higher prevalence of subtype I. In HIV-1 positive children we detected BKV DNA in 2 urine samples (6%) out of 33, both belonging to subtype I. The HIV-negative cluster did not show any positivity to BKV DNA. The results confirm a more frequent primary BKV infection in children of 3–5 years of age and a higher prevalence in hospitalized children affected by HIV-1. The most relevant finding was that among both the community and HIV-1 positive children the subtype I was the most frequently detected.
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- Detection of BK polyomavirus genotypes in healthy and HIV-positive children
European Journal of Epidemiology
Volume 13, Issue 6 , pp 653-657
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- BK virus
- Polymerase chain reaction
- Primary infection
- Restriction fragment length polymorphism assay
- Industry Sectors
- Author Affiliations
- 1. Institute of Microbiology, University ‘La Sapienza’, Rome, Italy
- 2. Institute of Hygiene, University ‘La Sapienza’, Rome, Italy
- 3. Virus Reference Division, Central Public Health Laboratory, London, UK
- 4. Department of Cellular & Developmental Biology, University ‘La Sapienza’, Rome, Italy