Journal of Neurocytology

, Volume 28, Issue 3, pp 169–185

Immunocytochemical localization of d-amino acid oxidase in rat brain


  • Sandra Moreno
    • Dipartimento di BiologiaUniversità Roma Tre
  • Roberta Nardacci
    • Dipartimento di Biologia Cellulare e dello SviluppoUniversità di Roma La Sapienza
  • AnnaMaria Cimini
    • Dipartimento di Biologia di Base ed ApplicataUniversità dell'Aquila, Coppito
  • Maria Paola Cer`
    • Dipartimento di Biologia di Base ed ApplicataUniversità dell'Aquila, Coppito

DOI: 10.1023/A:1007064504007

Cite this article as:
Moreno, S., Nardacci, R., Cimini, A. et al. J Neurocytol (1999) 28: 169. doi:10.1023/A:1007064504007


d-amino acid oxidase (d-AAO) is a peroxisomal flavoenzyme, the physiological substrate and the precise function of which are still unclear. We have investigated D-AAO distribution in rat brain, by immunocytochemistry, with an affinity-purified polyclonal antibody. Immunoreactivity occurred in both neuronal and glial cells, albeit at different densities. Glial immunostaning was strongest in the caudal brainstem and cerebellar cortex, particularly in astrocytes, Golgi-Bergmann glia, and tanycytes. Hindbrain neurons were generally more immunoreactive than those in the forebrain. Immunopositive forebrain cell populations included mitral cells in the olfactory bulb, cortical and hippocampal neurons, ventral pallidum, and septal, reticular thalamic, and paraventricular hypothalamic nuclei. Within the positive regions, not all the neuronal populations were equally immunoreactive; for example, in the thalamus, only the reticular and anterodorsal nuclei showed intense labelling. In the hindbrain, immunopositivity was virtually ubiquitous, and was especially strong in the reticular formation, pontine, ventral and dorsal cochlear, vestibular, cranial motor nuclei, deep cerebellar nuclei, and the cerebellar cortex, especially in Golgi and Purkinje cells.

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© Kluwer Academic Publishers 1999