Inflammation

, Volume 25, Issue 1, pp 17–23

Activity of the Leukocyte Nadph Oxidase in Whole Neutrophils and Cell-Free Neutrophil Preparations Stimulated with Long-Chain Polyunsaturated Fatty Acids

  • Susanne M. Schneider
  • Vivian S. Fung
  • Jan Palmblad
  • Bernard M. Babior
Article

DOI: 10.1023/A:1007019510569

Cite this article as:
Schneider, S.M., Fung, V.S., Palmblad, J. et al. Inflammation (2001) 25: 17. doi:10.1023/A:1007019510569

Abstract

Fish oils are known for their anti-inflammatory effects. In this paper we investigated the influence of eicosapentaenoic acid and docosahexaenoic acid (ω-3 fatty acids), as well as docosapentaenoic acid, a metabolic product of ω-3 fatty acid metabolism, on O2-production catalyzed by the NADPH oxidase in whole neutrophils and in a cell-free system consisting of neutrophil membranes and cytosol. As a standard we used arachidonic acid (an ω-6 fatty acid) found in a high proportion in the Western diet, and known as an effective activator of the oxidase in both systems. Our data show that with ω-3 fatty acids, the O2-production in both systems is reduced as compared to the effect of arachidonic acid. The effects are more pronounced with increasing carbon chain length and increasing numbers of double bonds. Our results suggest another mechanism besides the inhibition of eicosanoid and cytokine production to explain the beneficial effects of fish oils in reducing inflammation.

Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Susanne M. Schneider
    • 1
  • Vivian S. Fung
    • 1
  • Jan Palmblad
    • 2
  • Bernard M. Babior
    • 1
  1. 1.Department of Molecular and Experimental Medicine, Division of BiochemistryThe Scripps Research InstituteLa Jolla
  2. 2.Department of Medicine and Hematology, The Karolinska InstituteHuddinge University HospitalStockholmSweden