Molecular and Cellular Biochemistry

, Volume 200, Issue 1, pp 143–153

Sexual dimorphism in rat left atrial function and response to adrenergic stimulation

  • Dorie W. Schwertz
  • Vida Vizgirda
  • R. John Solaro
  • Mariann R. Piano
  • Connie Ryjewski
Article

DOI: 10.1023/A:1007011807383

Cite this article as:
Schwertz, D.W., Vizgirda, V., Solaro, R.J. et al. Mol Cell Biochem (1999) 200: 143. doi:10.1023/A:1007011807383

Abstract

A number of investigations in humans and animals suggest that there may be intrinsic sex-associated differences in cardiac function. Using left atrial preparations from male and female rat hearts, we examined differences in myocardial function and response to adrenergic agonists. Contractile parameters were measured in isolated atria by conventional isometric methods in the absence or presence of isoproterenol or phenylephrine. Responsiveness to Ca2+ was measured in detergent-skinned atrial fibers and actomyosin ATPase activity was measured in isolated myofibrils. Tetanic contractions were generated by treating the atrium with ryanodine followed by high frequency stimulation. Developed force was greater and maximal rates of contraction and relaxation were more rapid in the female atrium. The relationship between Ca2+ concentration and force in both intact atria and detergent-skinned atrial fibers in females fell to the left of that for males. At low Ca2+ concentrations, skinned fibers from female atria generated more force and myofibrils from female atria had higher myosin ATPase activity than males. Tetanic contraction in the presence of high extracellular Ca2+ was greater in female atria. Male atrium had larger inotropic responses to isoproterenol and to phenylephrine, but drug-elicited cAMP and inositol phosphate production did not differ between sexes. The results demonstrate sex-related differences in atrial function that can be partially explained by greater myofibrillar Ca2+-sensitivity in females. A potential contribution of sarcolemmal Ca2+ influx is suggested by greater tetanic contraction in ryanodine-treated female atrium. The larger response of males to adrenergic stimulation does not appear to be explained by higher production of relevant second messengers. Future studies will investigate the role of sex hormones in these sexually dimorphic responses and may indicate a need for gender-specific therapeutic interventions for myocardial dysfunction.

heartatriumratfunctionsex/genderisometric contractionforcecalcium sensitivitymyofibrillar ATPaseadrenergic agonistsisoproteronolphenylephrine

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Dorie W. Schwertz
    • 1
  • Vida Vizgirda
    • 1
  • R. John Solaro
    • 2
  • Mariann R. Piano
    • 1
  • Connie Ryjewski
    • 1
  1. 1.Department of Med. Surg. Nurs. and PharmacologyUniversity of Illinois M/C 802ChicagoUSA
  2. 2.Department of Physiology and BiophysicsUniversity of Illinois at ChicagoChicagoUSA