Molecular Biology Reports

, Volume 26, Issue 1, pp 29–34

Comparison of human COP9 signalosome and 26S proteasome ‘lid’

  • Wolfgang Henke
  • Katherine Ferrell
  • Dawadschargal Bech-Otschir
  • Michael Seeger
  • Rüdiger Schade
  • Peter Jungblut
  • Michael Naumann
  • Wolfgang Dubiel
Article

DOI: 10.1023/A:1006991419464

Cite this article as:
Henke, W., Ferrell, K., Bech-Otschir, D. et al. Mol Biol Rep (1999) 26: 29. doi:10.1023/A:1006991419464

Abstract

The human core COP9 signalosome consists of eight subunits which have been identified, cloned and sequenced. The components of COP9 signalosome possess homologies with eight non-ATPase regulatory subunits of the 26S proteasome. These polypeptides of the 19S regulator form a reversibly binding subcomplex called the ‘lid’. We isolated the ‘lid’ from human red blood cells and compared it with the COP9 signalosome complex. In addition to the non-ATPase regulatory polypeptides, we found a high molecular mass ATPase copurifying with the human ‘lid’. The COP9 signalosome-associated kinase activity is either not at all or only weakly affected by common kinase inhibitors such as 1-(5-Isoquinolinesulfonyl)-2-methyl-piperazine (H7), 5,6-dichloro-1-β-D-ribofuranosyl-benzimidazole (DRB) or Wortmannin. Curcumin, a tumor suppressor and effector of AP-1 activation, is a potent inhibitor of the COP9 signalosome kinase activity with a Ki of about 10 μM. Since curcumin is known as an inhibitor of the c-Jun N-terminal kinase (JNK) signaling pathway acting upstream of the MAP kinase kinase kinase level, one site of action of the COP9 signalosome might be proximal to regulators on that level.

COP9 signalosome26S proteasome19S regulatorJNKcurcumin

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Wolfgang Henke
    • 1
    • 2
  • Katherine Ferrell
    • 1
    • 2
  • Dawadschargal Bech-Otschir
    • 1
    • 2
  • Michael Seeger
    • 1
    • 2
  • Rüdiger Schade
    • 3
  • Peter Jungblut
    • 4
  • Michael Naumann
    • 4
  • Wolfgang Dubiel
    • 1
    • 2
  1. 1.Institute of BiochemistryGermany
  2. 2.Institute of Pharmacology and Toxicology, Medical Faculty CharitéHumboldt UniversityGermany
  3. 3.Abt. Molekulare BiologieMax-Planck-Institut für InfektionsbiologieBerlinGermany
  4. 4.Abt. Molekulare BiologieMax-Planck-Institut für InfektionsbiologieBerlinGermany