Molecular and Cellular Biochemistry

, Volume 194, Issue 1, pp 53–62

Interaction of cholera toxin and Escherichia coli heat-labile enterotoxin with glycoconjugates from rabbit intestinal brush border membranes: Relationship with ABH blood group determinants

  • Luis E. Balanzino
  • José L. Barra
  • Estela M. Galváin
  • German A. Roth
  • Clara G. Monferran
Article

DOI: 10.1023/A:1006971913175

Cite this article as:
Balanzino, L.E., Barra, J.L., Galváin, E.M. et al. Mol Cell Biochem (1999) 194: 53. doi:10.1023/A:1006971913175
  • 33 Downloads

Abstract

The capacity of cholera toxin (CT) and type I heat-labile enterotoxin produced by Escherichia coli isolated from human intestine (LTh) to interact with glycoconjugates bearing ABH blood group determinants from rabbit intestinal brush border membranes (BBM) was studied. On the basis of the type of intestinal compounds related to the human ABH blood group antigens, rabbits were classified as AB or H. Toxin binding to the intestinal glycolipids and glycoproteins depends on the blood group determinant borne by the glycoconjugate and on the analyzed toxin. LTh was capable of interacting preferentially with several blood group A- and B-active BBM glycolipids compared to those isolated from animals lacking these antigens (H rabbits). Also, LTh preferably bound to several BBM glycoproteins from AB rabbit intestines compared to those from H ones. One of these glycoproteins, the sucrase-isomaltase complex (EC 3.2.1.48-10) isolated from AB and H rabbits showed the same differential LTh binding. Conversely, CT practically did not recognize either blood group A-, B-, or H-active glycolipids and glycoproteins. These results may be relevant for carrying out in vivo experiments in rabbits in order to disclose the role of ABH active-glycoconjugates in the secretory response induced by LTh in rabbit intestine.

Escherichia coli heat-liable enterotoxin cholera toxin ABH blood groups intestinal brush border membranes rabbit intestine 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Luis E. Balanzino
    • 1
  • José L. Barra
    • 1
  • Estela M. Galváin
    • 1
  • German A. Roth
    • 1
  • Clara G. Monferran
    • 1
  1. 1.Departamento de Química Biológica 'Dr. Ranwel Caputto'- CIQUIBIC (CONICET), Facultad de Ciencias QuímicasUniversidad Nacional de CórdobaCórdobaArgentina

Personalised recommendations