Molecular and Cellular Biochemistry

, Volume 199, Issue 1, pp 93–102

Antioxidant properties of aspirin: Characterization of the ability of aspirin to inhibit silica-induced lipid peroxidation, DNA damage, NF-κB activation, and TNF-α production

  • Xianglin Shi
  • Min Ding
  • Zigang Dong
  • Fei Chen
  • Jiangping Ye
  • Suwei Wang
  • Stephen S. Leonard
  • Vince Castronova
  • Val Vallyathan
Article

DOI: 10.1023/A:1006934612368

Cite this article as:
Shi, X., Ding, M., Dong, Z. et al. Mol Cell Biochem (1999) 199: 93. doi:10.1023/A:1006934612368

Abstract

Electron spin resonance (ESR) was used to investigate the reaction of aspirin toward reactive oxygen species, such as hydroxyl radicals (·OH), superoxide radicals (O2-) and H2O2. The Fenton reaction (Fe(II) + H2O2 ---> FE(III) + -OH + OR) was used as a source of -OH radicals. The results show that aspirin is an efficient -OH radical scavenger with a reaction rate constant of k = 3.6 x 1010 M-1sec-1, which is faster than several well established antioxidants, such as ascorbate, glutathione and cysteine. However, aspirin is not a good scavenger for O2- or H2O2. Through its antioxidant property, aspirin exhibited a protective effect against silica-induced lipid peroxidation and DNA strand breakage. Aspirin also inhibited the activation of nuclear transcription factor-κb induced by silica, lipopolysaccharide or the transition metal, Fe(II), as demonstrated by electrophoretic mobility shift assay. The results show that aspirin functions as an antioxidant via its ability to scavenge -OH radicals. This antioxidant property may explain some of its various physiological and pharmacological actions.

aspirinantioxidant propertiessilicalipid peroxidationDNA damageNF-κBTNF-α

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Xianglin Shi
    • 1
  • Min Ding
    • 1
  • Zigang Dong
    • 2
  • Fei Chen
    • 1
  • Jiangping Ye
    • 1
  • Suwei Wang
    • 1
  • Stephen S. Leonard
    • 1
  • Vince Castronova
    • 1
  • Val Vallyathan
    • 1
  1. 1.Pathology and Physiology Research Branch, Health Effects Laboratory DivisionNational Institute for Occupational Safety and HealthMorgantownUSA
  2. 2.The Hormal InstituteUniversity of MinnesotaAustinUSA