Article

Molecular and Cellular Biochemistry

, Volume 196, Issue 1, pp 85-91

First online:

Oxidative stress in Systemic Sclerosis

  • Gabriele SimoniniAffiliated withDepartment of Pediatrics, University of Florence
  • , Marco Matucci CerinicAffiliated withDepartment of Medicine, Division of Rheumatology, University of Florence
  • , Sergio GeneriniAffiliated withDepartment of Medicine, Division of Rheumatology, University of Florence
  • , Massimo ZoppiAffiliated withDepartment of Medicine, Division of Rheumatology, University of Florence
  • , Mario AnichiniAffiliated withINRCA
  • , C. CesarettiAffiliated withINRCA
  • , Alberto PignoneAffiliated withDepartment of Medicine, Division of Rheumatology, University of Florence
  • , Fernanda FalciniAffiliated withDepartment of Pediatrics, University of Florence
  • , Torello LottiAffiliated withClinic of Dermatology, University of Florence
    • , Mario CagnoniAffiliated withDepartment of Pediatrics, University of Florence

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Abstract

In 63 patients affected by Systemic Sclerosis (SSc) (limited subset., 40; diffuse subset: 23; early: 30; advanced: 33) the peroxidation product diene-conjugates (DC) and antibodies against oxidised low density lipoproteins (Ab oxLDL) were tested in serum by a spectrophotometer (absorbance 234 mn) and by a standard ELISA respectively. The data were compared with those obtained by 21 healthy subjects. DC was significantly higher in patients (73.3 ± 37.2 μM/l; p < 0.0001) than in controls (48.4 ± 16.7) as well as in the limited (80 ± 48.8; p < 0.05) than in the diffuse subset (64.5 ± 36.4); and in early (84.1 ± 31.4; p < 0.05) than in advanced stage of the disease (67.9 ± 42.5). The levels Ab oxLDL were significantly higher in SSc patients (309.5 ± 367.2 mU/ml; p < 0.0001) in all its subsets (limited: 351.9 ± 351.1, p < 0.0001; diffuse: 207.7 ± 316. 1, p < 0.05; early: 428.9 ± 417.1, p < 0.001; advanced: 302.7 ± 89.9, p < 0.0001) than in controls (89.3 ± 29.1). These antibodies levels were higher in limited subset than in diffuse (p < 0.05) and in early SSc than in advanced SSc (p < 0.05). The highest values of parameters of oxidative stress are found in the early stages, when the episodes of reperfusion after ischemic episodes (Raynaud's phenomenon) are very ferequent. Moreover, the damage is higher in the early stages of SSc, with intact microvessels, than in late stages, when microvessels are very reduced in number, destroyed by the worsening of the disease. These radicals products works as well in other diseases such as myocardial ischemia and pulmonary fibrosis.These data show that the respiratory burst deduced their lipoperoxidation is higher in SSe than in controls, may be an important pathogenetic factors involved in tissue changes in SSe.

oxidative stress Systemic Sclerosis diene-conjugates antibodies against oxidised low density lipoproteins reperfusion injury