Molecular and Cellular Biochemistry

, Volume 196, Issue 1, pp 85–91

Oxidative stress in Systemic Sclerosis

  • Gabriele Simonini
  • Marco Matucci Cerinic
  • Sergio Generini
  • Massimo Zoppi
  • Mario Anichini
  • C. Cesaretti
  • Alberto Pignone
  • Fernanda Falcini
  • Torello Lotti
  • Mario Cagnoni
Article

DOI: 10.1023/A:1006922313774

Cite this article as:
Simonini, G., Matucci Cerinic, M., Generini, S. et al. Mol Cell Biochem (1999) 196: 85. doi:10.1023/A:1006922313774

Abstract

In 63 patients affected by Systemic Sclerosis (SSc) (limited subset., 40; diffuse subset: 23; early: 30; advanced: 33) the peroxidation product diene-conjugates (DC) and antibodies against oxidised low density lipoproteins (Ab oxLDL) were tested in serum by a spectrophotometer (absorbance 234 mn) and by a standard ELISA respectively. The data were compared with those obtained by 21 healthy subjects. DC was significantly higher in patients (73.3 ± 37.2 μM/l; p < 0.0001) than in controls (48.4 ± 16.7) as well as in the limited (80 ± 48.8; p < 0.05) than in the diffuse subset (64.5 ± 36.4); and in early (84.1 ± 31.4; p < 0.05) than in advanced stage of the disease (67.9 ± 42.5). The levels Ab oxLDL were significantly higher in SSc patients (309.5 ± 367.2 mU/ml; p < 0.0001) in all its subsets (limited: 351.9 ± 351.1, p < 0.0001; diffuse: 207.7 ± 316. 1, p < 0.05; early: 428.9 ± 417.1, p < 0.001; advanced: 302.7 ± 89.9, p < 0.0001) than in controls (89.3 ± 29.1). These antibodies levels were higher in limited subset than in diffuse (p < 0.05) and in early SSc than in advanced SSc (p < 0.05). The highest values of parameters of oxidative stress are found in the early stages, when the episodes of reperfusion after ischemic episodes (Raynaud's phenomenon) are very ferequent. Moreover, the damage is higher in the early stages of SSc, with intact microvessels, than in late stages, when microvessels are very reduced in number, destroyed by the worsening of the disease. These radicals products works as well in other diseases such as myocardial ischemia and pulmonary fibrosis.These data show that the respiratory burst deduced their lipoperoxidation is higher in SSe than in controls, may be an important pathogenetic factors involved in tissue changes in SSe.

oxidative stressSystemic Sclerosisdiene-conjugatesantibodies against oxidised low density lipoproteinsreperfusion injury

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Gabriele Simonini
    • 1
  • Marco Matucci Cerinic
    • 2
  • Sergio Generini
    • 2
  • Massimo Zoppi
    • 2
  • Mario Anichini
    • 3
  • C. Cesaretti
    • 3
  • Alberto Pignone
    • 2
  • Fernanda Falcini
    • 1
  • Torello Lotti
    • 4
  • Mario Cagnoni
    • 1
  1. 1.Department of PediatricsUniversity of FlorenceFlorenceItaly
  2. 2.Department of Medicine, Division of RheumatologyUniversity of FlorenceFlorenceItaly
  3. 3.INRCAFlorenceItaly
  4. 4.Clinic of DermatologyUniversity of FlorenceFlorenceItaly