Molecular and Cellular Biochemistry

, Volume 172, Issue 1, pp 67–79

Identification of proteins that interact with a protein of interest: Applications of the yeast two-hybrid system

Authors

  • R. Daniel Gietz
    • Department of Human GeneticsUniversity of Manitoba
  • Barbara Triggs-Raine
    • Department of Biochemistry and Molecular BiologyUniversity of Manitoba
  • Anne Robbins
    • Department of Human GeneticsUniversity of Manitoba
  • Kevin C. Graham
    • Department of Human GeneticsUniversity of Manitoba
  • Robin A. Woods
    • Department of BiologyUniversity of Winnipeg
Article

DOI: 10.1023/A:1006859319926

Cite this article as:
Gietz, R.D., Triggs-Raine, B., Robbins, A. et al. Mol Cell Biochem (1997) 172: 67. doi:10.1023/A:1006859319926

Abstract

The yeast two-hybrid system is a molecular genetic test for protein interaction. Here we describe a step by step procedure to screen for proteins that interact with a protein of interest using the two-hybrid system. This process includes, construction and testing of the bait plasmid, screening a plasmid library for interacting fusion proteins, elimination of false positives and deletion analysis of true positives. This procedure is designed to allow investigators to identify proteins and their encoding cDNAs that have a biologically significant interaction with your protein of interest.

protein interactiontwo-hybrid systemyeastscreeningfalse positive elimination

Copyright information

© Kluwer Academic Publishers 1997