Molecular and Cellular Biochemistry

, Volume 170, Issue 1, pp 43–51

Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells

Authors

  • Antonio D. McCarthy
    • Cátedra de Bioquímica Patólogica, Facultad de Ciencias ExactasUniversidad Nacional de La Plata
  • Susana B. Etcheverry
    • Cátedra de Bioquímica Patólogica, Facultad de Ciencias ExactasUniversidad Nacional de La Plata
  • Liliana Bruzzone
    • División Química Analítica, Facultad de Ciencias ExactasUniversidad Nacional de La Plata
  • Ana M. Cortizo
    • Cátedra de Bioquímica Patólogica, Facultad de Ciencias ExactasUniversidad Nacional de La Plata
Article

DOI: 10.1023/A:1006816223292

Cite this article as:
McCarthy, A.D., Etcheverry, S.B., Bruzzone, L. et al. Mol Cell Biochem (1997) 170: 43. doi:10.1023/A:1006816223292

Abstract

Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner.

advanced glycation endproductsdiabetes mellitusosteoblastic cellscell proliferationcell differentiationbone

Copyright information

© Kluwer Academic Publishers 1997