Clinical & Experimental Metastasis

, Volume 17, Issue 6, pp 501–506

A nude mouse model of human osteosarcoma lung metastases for evaluating new therapeutic strategies

  • Shu-Fang Jia
  • Laura L. Worth
  • Eugenie S. Kleinerman
Article

DOI: 10.1023/A:1006623001465

Cite this article as:
Jia, SF., Worth, L.L. & Kleinerman, E.S. Clin Exp Metastasis (1999) 17: 501. doi:10.1023/A:1006623001465

Abstract

The purpose of these studies was to develop a metastatic osteosarcoma nude mouse model to evaluate the in vivo efficacy of new therapeutic compounds. Human SAOS-2 osteosarcoma cells (106 cells) were injected i.v. into nude mice. Cells isolated from a rare pulmonary metastases 6 months later were established (SAOS-LM1) in culture and re-injected. This procedure was repeated 5 additional times to produce the SAOS-LM6 cell line. Visible pulmonary nodules were present 8 weeks following i.v. injection of 106 SAOS-LM6 cells as compared to 17 weeks using SAOS-LM2 cells. Microscopic SAOS-LM6 pulmonary metastases were demonstrated at 6 weeks. Administration of adriamycin on week 9 resulted in regression of macroscopic SAOS-LM6 lung tumors. The ability of the model to be used to evaluate the effectiveness of a biologic agent against microscopic disease was also verified. It was concluded that this model can assess therapeutic efficacy and therefore, may have a role in investigating the potential of novel approaches aimed at eliminating pulmonary metastatic osteosarcoma.

lung metastasismouse modelosteosarcoma

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Shu-Fang Jia
    • 1
  • Laura L. Worth
    • 2
  • Eugenie S. Kleinerman
    • 1
    • 2
  1. 1.Departments of Cancer BiologyThe University of TexasHoustonUSA
  2. 2.Department of PediatricsThe University of TexasHoustonUSA