Article

Clinical & Experimental Metastasis

, Volume 16, Issue 1, pp 50-61

First online:

αv Integrins mediate adhesion and migration of breast carcinoma cell lines

  • Nanette C. WongAffiliated withCancer Research Center, Program on Cell Adhesion and the Extracellular Matrix, The Burnham Institute
  • , Barbara M. MuellerAffiliated withDepartment of Immunology, The Scripps Research Institute
  • , Carlos F. BarbasAffiliated withDepartment of Molecular, The Scripps Research Institute
  • , Pete RuminskiAffiliated withDepartment of Cell Biology, The Scripps Research Institute
  • , Vito QuarantaAffiliated with
  • , Emme C. K. LinAffiliated withCancer Research Center, Program on Cell Adhesion and the Extracellular Matrix, The Burnham Institute
  • , Jeffrey W. SmithAffiliated withCancer Research Center, Program on Cell Adhesion and the Extracellular Matrix, The Burnham Institute

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Abstract

Integrins with the αv subunit are involved in cell adhesion and cellular signaling. Some αv integrins have been associated with tumor progression and dissemination. The objective of this study was to assess the contribution of αv integrins to the adhesive and migratory behavior of cells derived from breast carcinoma (BCA). The expression and function of αv integrins was characterized in three BCA cell lines which exhibit different metastatic potentials. These include MCF-7 cells which metastasize inefficiently, MDA-MB-231 cells, which have a moderate metastatic potential, and MDA-MB-435 cells, which metastasize extensively. Each cell type displays a different repertoire of αv integrins on the cell surface. The complement of αv integrins on each cell type influences their ability to adhere and migrate. The most striking difference among these cell lines was the expression of the αvβ3 3 integrin. The highly metastatic MDA-MB-435 cells express substantial levels of this receptor, whereas MDA-MB-231 and MCF-7 cells do not. The MDA-MB-435 cells showed a greater ability to adhere and to migrate and this functional difference can largely be attributed to the expression of αvβ3 integrin. This characterization is a first step toward determining the role of αv integrins in animal models of BCA metastasis, and lends support to the hypothesis that the αvβ3 integrin can be a contributing factor in metastatic disease. © Rapid Science 1998

adhesion integrin invasion metastasis migration