Plant Molecular Biology

, Volume 43, Issue 2, pp 361–376

Use of matrix attachment regions (MARs) to minimize transgene silencing

  • George C. Allen
  • Steven Spiker
  • William F. Thompson
Article

DOI: 10.1023/A:1006424621037

Cite this article as:
Allen, G.C., Spiker, S. & Thompson, W.F. Plant Mol Biol (2000) 43: 361. doi:10.1023/A:1006424621037

Abstract

Matrix attachment regions (MARs) are operationally defined as DNA elements that bind specifically to the nuclear matrix in vitro. It is possible, although unproven, that they also mediate binding of chromatin to the nuclear matrix in vivo and alter the topology of the genome in interphase nuclei. When MARs are positioned on either side of a transgene their presence usually results in higher and more stable expression in transgenic plants or cell lines, most likely by minimizing gene silencing. Our review explores current data and presents several plausible models to explain MAR effects on transgene expression.

chromatin structure gene silencing MAR nuclear matrix nuclear scaffold SAR 

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • George C. Allen
    • 1
  • Steven Spiker
    • 2
  • William F. Thompson
    • 1
    • 2
    • 3
  1. 1.Department of Botany
  2. 2.Department of GeneticsUSA
  3. 3.Department of Crop ScienceNorth Carolina State UniversityRaleighUSA

Personalised recommendations