Plant Cell, Tissue and Organ Culture

, Volume 63, Issue 1, pp 67–72

Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis

Authors

  • Park So Young
    • Research Center for the Development of Advanced Horticultural TechnologyChungbuk National University
  • H.N. Murthy
    • Research Center for the Development of Advanced Horticultural TechnologyChungbuk National University
    • Research Center for the Development of Advanced Horticultural TechnologyChungbuk National University
Article

DOI: 10.1023/A:1006420116883

Cite this article as:
So Young, P., Murthy, H. & Kee Yoeup, P. Plant Cell, Tissue and Organ Culture (2000) 63: 67. doi:10.1023/A:1006420116883

Abstract

Protocorm-like bodies (PLBs) formed on leaf segmentsin vitro were used as explants for bioreactor cultures. Continuous immersion cultures (air lift column and air lift-balloon bioreactor), and temporary immersion cultures (with or without charcoal filter attached) were used for the culture of PLB sections. A temporary immersion culture with charcoal filter attached was most suitable for PLB culture. About 18,000 PLBs were harvested from 20 g of inoculum (∼1000 PLB sections) in 2 l Hyponex medium after 8 weeks of incubation. Aeration in a bioreactor at 0.5 or 2.0 volume of air per volume of medium min−1 (vvm) yielded similar levels of biomass production. PLBs grown in bioreactors were cultured on solid Murashige and Skoog, Vacin and Went, Knudson C, Lindemann and Hyponex media. Hyponex medium was found to be suitable for conversion of PLBs into plantlets and 83% of PLBs transformed into plantlets on this medium. The feasibility of using PLBs for large-scale micropropagation was evaluated for scaled-up liquid cultures in bioreactors, rate of proliferation, and regeneration.

bioreactorliquid culturesmicropropagationorchid

Copyright information

© Kluwer Academic Publishers 2000