Plant Molecular Biology

, Volume 42, Issue 3, pp 531–544

Male-sterile tobacco displays abnormal mitochondrial atp1 transcript accumulation and reduced floral ATP/ADP ratio

  • Per Bergman
  • Johan Edqvist
  • Isabelle Farbos
  • Kristina Glimelius
Article

DOI: 10.1023/A:1006388814458

Cite this article as:
Bergman, P., Edqvist, J., Farbos, I. et al. Plant Mol Biol (2000) 42: 531. doi:10.1023/A:1006388814458

Abstract

Alloplasmic male sterility is commonly obtained in Nicotiana by combining the nucleus from N. tabacum with the cytoplasm from other Nicotiana species. Besides being male-sterile, most of these cultivars also display changes in floral organ structure. Flowers from male-sterile plants containing the nucleus from N. tabacum combined with N. repanda cytoplasm develop stamen with shortened filaments and shrivelled anthers capped with stigmatoids. Male fertility and normal floral development can be restored by introduction of a restorer chromosome fragment from the cytoplasmic donor N. repanda into the N. tabacum nucleus. A novel reading frame, orf274, located upstream of atp1 in the mitochondrial genomes of both N. tabacum and N. repanda, as well as in the male-sterile and fertility-restored plants was identified. Co-transcripts of orf274 and atp1 were detected by RT-PCR in all four cultivars, but these transcripts accumulate to levels detectable by northern hybridization only in male-sterile plants. These co-transcripts neither generate detectable levels of an ORF274 polypeptide nor lead to altered expression of the ATP synthase subunit α. Measurement of ATP and ADP steady-state levels, however, revealed that the ATP/ADP ratio is significantly lower in young floral buds of male-sterile plants than in fertile plants.

ATPatp1cytoplasmic male sterilityflower developmentmitochondria

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Per Bergman
    • 1
  • Johan Edqvist
    • 1
  • Isabelle Farbos
    • 1
  • Kristina Glimelius
    • 1
  1. 1.Department of Plant Biology, Uppsala Genetic CenterSwedish University of Agricultural SciencesUppsalaSweden