Article

Plant Molecular Biology

, Volume 41, Issue 5, pp 669-677

First online:

Comparative differential RNA display analysis of arbuscular mycorrhiza in Pisum sativum wild type and a mutant defective in late stage development

  • Laurence LapopinAffiliated withMax-Planck-Institut für terrestrische Mikrobiologie, and, Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Karl-von-Frisch-Strasse
  • , Vivienne Gianinazzi-PearsonAffiliated withLaboratoire de Phytoparasitologie INRA/CNRS, CMSE-INRA
  • , Philipp FrankenAffiliated withMax-Planck-Institut für terrestrische Mikrobiologie, and, Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Karl-von-Frisch-Strasse

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Abstract

In order to analyse gene expression associated with the late stages of arbuscular mycorrhizal development between Pisum sativum and Glomus mosseae, comparative differential RNA display was carried out using wild-type P. sativum and a mutant, RisNod24, where the fungal partner is not able to form functional arbuscules. Comparison of RNA accumulation patterns between controls, G. mosseae-colonized mutant and wild-type roots resulted in the identification of four differentially occurring cDNA fragments. One of the corresponding genes was from the fungus and three of plant origin. One plant gene, Psam4 (P. sativum arbuscular mycorrhiza-regulated), was analysed in more detail. Sequencing of a cDNA clone showed that Psam4 encodes a proline-rich protein. Northern blot analysis and quantitative RT-PCR revealed a higher basal level of Psam4 RNA accumulation in the mutant compared to the wild type. In both pea genotypes, RNA accumulation was reduced after inoculation with mycorrhiza- or nodule-forming symbiotic microorganisms, but enhanced after infection with a root pathogenic fungus.

Aphanomyces euteiches arbuscular mycorrhiza differential RNA display Glomus mosseae Pisum sativum proline-rich protein Rhizobium leguminosarum