Breast Cancer Research and Treatment

, Volume 59, Issue 2, pp 163–170

Lipid peroxidation, free radical production and antioxidant status in breast cancer

  • Gibanananda Ray
  • Sanjay Batra
  • Nootan Kumar Shukla
  • Suryanarayan Deo
  • Vinod Raina
  • Seetharaman Ashok
  • Syed Akhtar Husain
Article

DOI: 10.1023/A:1006357330486

Cite this article as:
Ray, G., Batra, S., Shukla, N.K. et al. Breast Cancer Res Treat (2000) 59: 163. doi:10.1023/A:1006357330486

Abstract

Reactive oxygen metabolites (ROMs), including superoxide anion (O2·−), hydrogen peroxide (H2O2) and hydroxyl radical (·OH), play an important role in carcinogenesis. There are some primary antioxidants such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) which protect against cellular and molecular damage caused by the ROMs. We conducted the present study to determine the rate of O2·− and H2O2 production, and concentration of malondialdehyde (MDA), as an index of lipid peroxidation, along with the SOD, GPx and CAT activities in 54 breast cancer (BC) patients. Forty-two age- and sex-matched patients with minor surgical problems, who had no history of any neoplastic or breast disorders, were taken as controls.

The rate of O2·− production was significantly higher (p<0.001) in BC patients than controls, irrespective of clinical stages and menopausal status. Similarly, H2O2 production was significantly higher in BC patients, especially in stage III and postmenopausal groups, as compared to the respective controls. MDA concentration was also observed significantly elevated in stage II (p<0.001), stage III (p<0.01), postmenopausal (p<0.005), and premenopausal (p<0.02) group as compared to their corresponding controls. SOD and GPx activities were found significantly raised in all the groups (p<0.001), except the GPx activity was found a smaller alteration in stage IV (p<0.02). On the contrary, CAT activity was found significantly depressed in all the study groups. The maximum depression was observed in stage II (−61.8%). Lower CAT activity in our study may be the effect of higher production of ROMs, particularly O2·− and ·OH. SOD and GPx, however, were less effected by these higher ROMs production. The results of our study have shown a higher ROMs production and decreased CAT activity, which support the oxidative stress hypothesis in carcinogenesis. The relatively higher SOD and GPx may be due to the response of increased ROMs production in the blood. However, the higher SOD and GPx activities may be inadequate to detoxify high levels of H2O2 into H2O leading to the formation of the most dangerous ·OH radical followed by MDA. Therefore, administration of CAT may be helpful in the management of BC patients. However, further elaborate clinical studies are required to evaluate the role of such antioxidant enzymes in BC management.

breast cancercatalaseglutathione peroxidasemalondialdehydereactive oxygen metabolitessuperoxide dismutase

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Gibanananda Ray
    • 1
  • Sanjay Batra
    • 2
  • Nootan Kumar Shukla
    • 3
  • Suryanarayan Deo
    • 3
  • Vinod Raina
    • 4
  • Seetharaman Ashok
    • 5
  • Syed Akhtar Husain
    • 1
  1. 1.Department of BiosciencesJamia Millia IslamiaNew DelhiIndia
  2. 2.Department of BiochemistryKalawati Saran Children's HospitalNew DelhiIndia
  3. 3.Department of Surgical Oncology, and Department of Medical OncologyNew DelhiIndia
  4. 4.Department of Medical OncologyInstitute of Rotary Cancer Hospital, All India Institute of Medical SciencesNew DelhiIndia
  5. 5.Department of SurgeryLady Hardinge Medical College and Associated Hospitals, c[New DelhiIndia