The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
We have expressed the gene (PAB1) encoding the yeast polyadenylate-binding protein (Pab1p) in tobacco. Plants that accumulate the Pab1p display a range of abnormalities, ranging from a characteristic chlorosis in leaves to a necrosis and large inhibition of growth. The severity of these abnormalities reflects the levels of yeast Pab1p expression in the transgenic plants. In contrast, no obvious differences could be seen in callus cultures between the transgene and vector control. Plants that display PAB-associated abnormalities were resistant to a range of plant pathogens, and had elevated levels of expression of a pathogenesis-related gene. These two properties – impairment of growth and induction of defense responses – indicate that the yeast PAB1 gene can act as a disease lesion mimic gene in plants.
- Abad, M.S., Hakimi, S.M., Kaniewski, W.K., Rommens, C.M.T., Shulaev, V., Lam, E. and Shah, D.M. 1997. Characterization of acquired resistance in lesion-mimic transgenic potato expressing bacterio-opsin. Mol. Plant-Microbe Interact. 10: 635–645.
- Amrani, N., Minet, M., Le Gouar, M., Lacroute, F. and Wyers, F. 1997. Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro. Mol. Cell. Biol. 17: 3694–3701.
- Bandziulis, R.J., Swanson, M.S. and Dreyfuss, G. 1989. RNAbinding proteins as developmental regulators. Genes Dev. 3: 431–436.
- Becker, F., Buschfeld, E., Schell, J. and Bachmair, A. 1993. Altered response to viral infection by tobacco plants perturbed in ubiquitin system. Plant J. 3: 875–881.
- Beffa, R., Szell, M., Meuwly, P., Pay, A., Voegeli-Lange, R., Metraux, J.P., Neuhaus, G., Meins, J.F. Jr. and Nagy, F. 1995. Cholera toxin elevates pathogen resistance and induces pathogenesis-related gene expression in tobacco. EMBO J. 14: 5753–5761.
- Belostotsky, D.A. and Meagher, R.B. 1993. Differential organspecific expression of three poly(A)-binding-protein genes from Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 90: 6686–6690.
- Belostotsky, D.A. and Meagher, R.B. 1996. A pollen-, ovule-and early embryo-specific poly(A) binding protein from Arabidopsis complements essential functions in yeast. Plant Cell 8: 1261–1275.
- Bernstein, P. and Ross, J. 1989. Poly(A), poly(A) binding protein and the regulation of mRNA stability. Trends Biochem. Sci. 14: 373–377.
- Cornelissen, B.J.C., Horowitz, J., van Kan, J.A.L., Goldberg, R.B. and Bol, J.F. 1987. Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group. Nucl. Acids Res. 15: 6799–6811.
- Dangl, J.L., Dietrich, R.A. and Richberg, M.H. 1996. Death don't have no mercy: cell death programs in plant-microbe interactions. Plant Cell 8: 1793–1807.
- De Melo Neto, O.P., Standart, N. and Martins de Sa, C. 1995. Autoregulation of poly(A)-binding protein synthesis in vitro. Nucl. Acids Res. 23: 2198–2205.
- Haynes, S.R. 1992. The RNA motif protein family. New Biol. 4: 421–429.
- Herbers, K., Meuwly, P., Frommer, W.B., Metraux, J.-P. and Sonnewald, U. 1996. Systemic acquired resistance mediated by ectopic expression of invertase: possible hexose sensing in the secretory pathway. Plant Cell 8: 793–803.
- Hilson, P., Carroll, K.L. and Masson, P.H. 1993. Molecular characterization of PAB2, a member of the multigene family coding for poly(A)-binding proteins in Arabidopsis thaliana. Plant Physiol. 103: 525–533.
- Horsch, R.B., Fry, J.E., Hoffmann, N.L., Eichholtz, D., Rogers, S.G. and Fraley, R.T. 1985. A simple and general method for transferring genes to plants. Science 227: 1229–1231.
- Le, H., Chang, S.C., Tanguay, R.L. and Gallie, D.R. 1997. The wheat poly(A)-binding protein functionally complements pab1 in yeast. Eur. J. Biochem. 243: 350–357.
- Le, H., Tanguay, R.L., Balasta, M.L., Wie, C.C., Browning, K.S., Metz, A.M., Goss, D.J. and Gallie, D.R. 1997. Translation initiation factors eIF-iso4G and eIF-4B interact with the poly(A)-binding protein and increase its RNA binding activity. J. Biol. Chem. 272: 16247–16255.
- Maiti, I.B., Murphy, J.F., Shaw, J.G. and Hunt, A.G. 1993. Plants that express a potyvirus proteinase gene are resistant to virus infection. Proc. Natl. Acad. Sci. USA 90: 6110–6114.
- Marhoul, J.F. and Adams, T.H. 1996. Aspergillus fabM encodes an essential product that is related to poly(A)-binding protein and activates development when overexpressed. Genetics 144: 1463–1470.
- Matzke, M. and Matzke, A.J.M. 1993. Genomic imprinting in plants: parental effects and trans-inactivation phenomena. Annu. Rev. Plant Physiol. Plant Mol. Biol. 44: 53–76.
- Minvielle-Sebastia, L., Preker, P.J., Wiederkehr, T., Strahm, Y. and Keller, W. 1997. The major yeast poly(A) binding protein is associated with cleavage factor IA and functions in premessenger RNA 30 end formation. Proc. Natl. Acad. Sci. USA 94: 7897–7902.
- Mittler, R., Shulaev, V. and Lam, E. 1995. Coordinated activation of programmed cell death and defense mechanisms in transgenic tobacco plants expressing a bacterial proton pump. Plant Cell 7: 29–42.
- Mogen, B.D., MacDonald, M.H., Leggewie, G. and Hunt, A.G. 1992. Several distinct types of sequence elements are required for efficient mRNA 30 end formation in a pea rbcS gene. Mol. Cell. Biol. 12: 5406–5414.
- Sachs, A.B. and Davis, R.W. 1989. The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunitdependent translation initiation. Cell 58: 857–867.
- Sachs, A.B., Davis, R.W. and Kornberg, R.D. 1987. A single domain of yeast poly(A)-binding protein is necessary and suf-ficient for RNA binding and cell viability. Mol. Cell. Biol. 7: 3268–3276.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
- Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463–5467.
- Sano, H., Seo, S., Orudgev, E., Youssefian, S., Ishizuka, K. and Ohashi, Y. 1994. Expression of the gene for a small GTP binding protein in transgenic tobacco elevates endogenous cytokinin levels, abnormally induces salicylic acid in response to wounding and increases resistance to tobacco mosaic virus infection. Proc. Natl. Acad. Sci. USA 91: 10556–10560.
- Schardl, C., Byrd, A.D., Benzion, G.B., Altschuler, M.A., Hildebrand, D.F. and Hunt, A.G. 1987. Design and construction of a versatile system for the expression of foreign genes in plants. Gene 61: 1–11.
- Sieliwanowicz, B. 1987. The influence of poly(A)-binding proteins on translation of poly(A)C RNA in a cell-free system from embryo axes of dry pea seeds. Biochim. Biophys. Acta 908: 54–59.
- Tadege, M., Bucher, M., Stähli, W., Suter, M., Dupuis, I. and Kuhlemeier, C. 1998. Activation of plant defense responses and sugar efflux by expression of pyruvate decarboxylase in potato leaves. Plant J. 16: 661–672.
- Terryn, N., Neyt, P., De Clerq, R., De Keyser, A., Van Den Daele, H., Ardiles, W., Dehais, P., Rouze, P., Gielen, J., Villarroel, R. and Van Montagu, M. 1997. Sequence analysis of a 24-kb contiguous region at the Arabidopsis thaliana PFL locus on chromosome 1. FEBS Lett. 416: 156–160.
- Towbin, H., Staehelin, T. and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76: 4350–4354.
- Wei, C.C., Balasta, M.L., Ren, J. and Goss, D.J. 1998. Wheat germ poly(A) binding protein enhances the binding affinity of eukaryotic initiation factor 4F and (iso)4F for cap analogues. Biochemistry 37: 1910–1916.
- Yang, J. and Hunt, A.G. 1992. Purification and characterization of a 70-kilodalton polyadenylate-binding protein from pea (Pisum sativum). Plant Physiol. 98: 1115–1120.
- Zambryski, P., Joos, H., Gentello, C., Leemans, J., Van Montagu, M. and Schell, J. 1983. Ti-plasmid vector for the introduction of DNA into plant cells without altering their normal regeneration capacity. EMBO J. 2: 2143–2150.
- The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants
Plant Molecular Biology
Volume 42, Issue 2 , pp 335-344
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- disease resistance
- lesion mimic
- poly(A)-binding proteins
- posttranscriptional control
- Industry Sectors