Breast Cancer Research and Treatment

, Volume 58, Issue 2, pp 137–140

Mammaglobin B as a novel marker for detection of breast cancer micrometastases in axillary lymph nodes by reverse transcription‐polymerase chain reaction

  • Tomohiko Aihara
  • Yoshiyuki Fujiwara
  • Masaru Ooka
  • Isao Sakita
  • Yasuhiro Tamaki
  • Morito Monden
Article

DOI: 10.1023/A:1006335817889

Cite this article as:
Aihara, T., Fujiwara, Y., Ooka, M. et al. Breast Cancer Res Treat (1999) 58: 137. doi:10.1023/A:1006335817889

Abstract

A novel reverse transcription‐polymerase chain reaction (RT‐PCR) assay using mammaglobin B gene was developed for detection of breast cancer micrometastases in axillary lymph nodes. Fourteen primary breast cancers and 56 axillary lymph nodes from six patients with primary breast cancer and 15 control lymph nodes from non‐cancer bearing patients were subjected to this assay. The transcript of mammaglobin B gene was detected in none of the control lymph nodes, but in all of the 14 primary breast cancers. Eleven out of the 56 lymph nodes from the patients, which were shown to be positive by histological examination, were also proven positive by this assay. On the other hand, fourteen of the 45 (31%) histologically negative lymph nodes were also shown to express mammaglobin B mRNA, which suggested the presence of micrometastases in these lymph nodes. RT‐PCR using mammaglobin B gene could therefore be a useful tool for detection of micrometastases of breast cancer.

diagnosis gene transcript micrometastasis molecular marker reverse transcription‐polymerase chain reaction 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Tomohiko Aihara
    • 1
  • Yoshiyuki Fujiwara
    • 1
  • Masaru Ooka
    • 1
  • Isao Sakita
    • 1
  • Yasuhiro Tamaki
    • 1
  • Morito Monden
    • 1
  1. 1.Department of Surgery IIOsaka University Medical SchoolSuita, OsakaJapan