Plant Molecular Biology

, Volume 40, Issue 2, pp 355–363

Expression and cellular localization of Atrab28 during Arabidopsis embryogenesis

Authors

  • César Arenas-Mena
    • Departament de Genètica MolecularCentre d'Investigació i Desenvolupament, C.S.I.C.
  • Monique Raynal
    • Laboratoire de Physiologie et Biologie Moléculaire des Plantes, UMR 5545 CNRSUniversité de Perpignan
  • Antonio Borrell
    • Laboratoire de Physiologie et Biologie Moléculaire des Plantes, UMR 5545 CNRSUniversité de Perpignan
  • Fabrice Varoquaux
    • Laboratoire de Physiologie et Biologie Moléculaire des Plantes, UMR 5545 CNRSUniversité de Perpignan
  • Mari Cruz Cutanda
    • Instituto de Biología Molecular y Celular de PlantasUniversidad Politécnica de Valencia-C.S.I.C.
  • Robin A.P. Stacy
    • Instituto de Biología Molecular y Celular de PlantasUniversidad Politécnica de Valencia-C.S.I.C.
  • Montserrat Pagès
    • Departament de Genètica MolecularCentre d'Investigació i Desenvolupament, C.S.I.C.
  • Michel Delseny
    • Laboratoire de Physiologie et Biologie Moléculaire des Plantes, UMR 5545 CNRSUniversité de Perpignan
  • Francisco A. Culiáñez-Macià
    • Instituto de Biología Molecular y Celular de PlantasUniversidad Politécnica de Valencia-C.S.I.C.
Article

DOI: 10.1023/A:1006219315562

Cite this article as:
Arenas-Mena, C., Raynal, M., Borrell, A. et al. Plant Mol Biol (1999) 40: 355. doi:10.1023/A:1006219315562

Abstract

The maize abscisic acid (ABA)-responsive gene rab28 has been shown to be ABA-inducible in embryos and vegetative tissues, expression being mostly restricted to vascular elements during late embryogenesis. In the course of an expressed sequence tags (ESTs) programme, we have isolated an Arabidopsis thaliana gene, Atrab28, encoding the orthologue of maize rab28. The Atrab28 cDNA is 1090 bp long, including a poly(A)+ stretch, and encodes a polypeptide of 262 amino acids. Atrab28 antibody against the recombinant protein recognizes a polipeptide of about 30 kDa and pI 6, in close agreement with the predicted molecular mass and pI. As for maize rab28, expression studies with Atrab28 revealed high specificity for embryo tissues, transcription being stimulated by the transcriptional activator abi3. In contrast, Atrab28 was not induced in vegetative tissues by ABA, osmotic stress or dehydration. The expression of Atrab28 mRNA and the accumulation of Atrab28 protein was largely restricted to provascular tissues of mature embryos and in the seed coat outer tegument and embryo and silique epidermis, as revealed by in situ hybridization and immunocytochemistry with anti-Atrab28 antibodies.

Arabidopsis thalianaembryo-specific gene expressionLea proteins

Copyright information

© Kluwer Academic Publishers 1999