Plant Cell, Tissue and Organ Culture

, Volume 54, Issue 3, pp 153–159

An optimising protocol for protoplast regeneration of three peppermint cultivars ( Mentha x piperita)

  • Frédéric Jullien
  • Florence Diemer
  • Monique Colson
  • Olivier Faure
Article

DOI: 10.1023/A:1006185103897

Cite this article as:
Jullien, F., Diemer, F., Colson, M. et al. Plant Cell, Tissue and Organ Culture (1998) 54: 153. doi:10.1023/A:1006185103897

Abstract

An efficient protocol for plant regeneration from protoplasts of peppermint ‘Mitcham Digne 38’, ‘Mitcham Ribecourt 19’ and ‘Todd's#x2019; was developed by stepwise optimization of first cell division, microcalli formation and shoot differentiation. The rate of first cell divisions was strongly dependent on the addition of 2,4-D to callus induction medium. Best results were obtained with 1 μM 2,4-D in combination with NAA (2.5 μM) and BA (4 μM). Although liquid medium was more efficient to support first protoplast divisions, solid medium was clearly more suitable to sustain subsequent cell divisions leading to the formation of microcalli. Shoot organogenesis was induced from protoplast-derived calli by using reduced auxin concentration (0.5 μM NAA) and high concentration of cytokinins. Addition of 2.3 μM thidiazuron increased bud formation, allowing a regeneration frequency of more than 50% from calli of ‘Mitcham Digne 38’ and ‘Todd's’. Genotypic differences were noticed for regeneration capability and the pathway of shoot regeneration.

Labiatae thidiazuron 

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Frédéric Jullien
    • 1
  • Florence Diemer
    • 1
  • Monique Colson
    • 1
  • Olivier Faure
    • 1
  1. 1.Laboratoire de Biotechnologies Végétales Appliquées aux Plantes Aromatiques et MédicinalesSaint-Étienne Cédex 2France (Fax

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