Plant Molecular Biology

, Volume 39, Issue 3, pp 427–436

Identification of genes specifically expressed in cauliflower reproductive meristems. Molecular characterization of BoREM1

  • José Manuel Franco-Zorrilla
  • Begoña Fernández-calvín
  • Francisco Madueño
  • Marilyn Cruz-Alvarez
  • Julio Salinas
  • José Miguel martínez-zapater
Article

DOI: 10.1023/A:1006130629100

Cite this article as:
Franco-Zorrilla, J.M., Fernández-calvín, B., Madueño, F. et al. Plant Mol Biol (1999) 39: 427. doi:10.1023/A:1006130629100

Abstract

Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts.

reproductive development gene expression subtractive hybridization cauliflower 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • José Manuel Franco-Zorrilla
    • 2
  • Begoña Fernández-calvín
    • 1
  • Francisco Madueño
    • 3
  • Marilyn Cruz-Alvarez
    • 4
  • Julio Salinas
    • 1
  • José Miguel martínez-zapater
    • 1
  1. 1.Departamento de Mejora Genética y BiotecnologíaInstituto Nacional de Investigación y Tecnología Agraria y AlimentariaMadridSpain
  2. 2.Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Campus de laUniversidad Autónoma de Madrid, CantoblancoMadridSpain
  3. 3.Instituto de Biología Molecular y Celular de PlantasUniversidad Politécnica de Valencia-CSICValenciaSpain
  4. 4.Department of BioscienceSalem-Teikyo UniversitySt., SalemUSA

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