Plant Molecular Biology

, Volume 39, Issue 4, pp 785–795

Enhanced expression and differential inducibility of soybean chalcone synthase genes by supplemental UV-B in dark-grown seedlings

Authors

  • Takeshi Shimizu
    • Plant Breeding Laboratory, Faculty of AgricultureHirosaki University
  • Shinji Akada
    • Gene Research CenterHirosaki University
  • Mineo Senda
    • Gene Research CenterHirosaki University
  • Ryuji Ishikawa
    • Plant Breeding Laboratory, Faculty of AgricultureHirosaki University
  • Takeo Harada
    • Plant Breeding Laboratory, Faculty of AgricultureHirosaki University
  • Minoru Niizeki
    • Plant Breeding Laboratory, Faculty of AgricultureHirosaki University
  • Shyam K. Dube
    • Center for Agricultural BiotechnologyUniversity of Maryland Biotechnology Institute
Article

DOI: 10.1023/A:1006124219945

Cite this article as:
Shimizu, T., Akada, S., Senda, M. et al. Plant Mol Biol (1999) 39: 785. doi:10.1023/A:1006124219945

Abstract

By developing gene-specific RT-PCR and using filters to allow transmission down to 290 nm (UV-B+) or blocking all radiation below 320 nm (UV-B−), the effect of UV-B+ and UV-B− light on expression of each of the presently known seven members of soybean chalcone synthase (CHS) gene family in dark-grown seedlings was analyzed. Dark expression was detectable already in 18 h dark-germinating embryos, with progressive increases on successive days, suggesting that chs belongs to a class of genes expressed very early during germination, and that the expression at this stage is either constitutive or induced by non-light-dependent factors present in the seed or made available following imbibition. Exposure of 18 h dark-germinating embryos to UV-B− or to UV-B+ light did not lead to an increase in chs signal. However, the 24 h dark-germinating embryos showed a distinct effect of UV-B+, interestingly coinciding with the stage when the head of seedlings was in the process of being pushed up above ground by stem elongation, suggesting the possibility of a developmental switch modulating the appearance of UV-B response. The response to UV-B− was most prominent in chs1 and almost silent in chs2, while the up-regulation by UV-B+ was most prominent in chs5 and chs6 and much less so in chs2. Interestingly, chs2 was noted to be the only member of the Gmchs gene family devoid of H-box, raising the possibility that the H-box may be a good indicator of the photo-inducibility of a chs gene.

UV-Bsoybeanchalcone synthasegene expression

Copyright information

© Kluwer Academic Publishers 1999