Breast Cancer Research and Treatment

, Volume 54, Issue 3, pp 235–244

Tissue inhibitor of metalloprotease (TIMP)‐1 and proliferative behaviour of clonal breast cancer cells

  • Claudio Luparello
  • Grazia Avanzato
  • Cintia Carella
  • Ida Pucci‐Minafra
Article

DOI: 10.1023/A:1006121129382

Cite this article as:
Luparello, C., Avanzato, G., Carella, C. et al. Breast Cancer Res Treat (1999) 54: 235. doi:10.1023/A:1006121129382

Abstract

In the present paper, we have examined whether human tissue inhibitor of metalloprotease‐1 (hTIMP‐1) is able to exert a growth factor‐like effect on two clonal cell lines (BC‐3A and BC‐61), isolated from a parental line of human breast carcinoma cells (8701‐BC), and endowed with different growth and invasive behaviour ‘in vitro’ and in nude mouse. The data obtained indicate that only the more tumorigenic clonal cell line (BC‐61) is responsive to hTIMP‐1 treatment by increasing its proliferative rate in a dose‐dependent manner. It was also found that BC‐61 cells selectively express a transmembrane protein of about 80 kDa able to bind hTIMP‐1 ‘in vitro’ and ‘in vivo’ with high affinity (Kd of 0.07 ± 0.004   nM), and that treatment of BC‐61 cells with a proliferation‐promoting concentration of hTIMP‐1 is able to stimulate tyrosine‐targeted phosphorylation. The cumulative results obtained strongly support the hypothesis that hTIMP‐1, ‘classically’ regarded as a collagenase inhibitor, may be a crucial element of the extracellular signalling network during breast cancer development by controlling cell growth phenotype in autocrine and paracrine manner, and that intratumoural heterogeneity for the biological response to TIMP‐1 may exist within the composite cell population of the primary tumour site.

breast cancer cell culture cell growth TIMP‐1 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Claudio Luparello
    • 1
  • Grazia Avanzato
    • 1
  • Cintia Carella
    • 1
  • Ida Pucci‐Minafra
    • 1
  1. 1.Dipartimento di Biologia Cellulare e dello Sviluppo, UniversitàPalermoItaly