A novel protein associated with citrus blight has sequence similarities to expansin Article DOI:
Cite this article as: Ceccardi, T.L., Barthe, G.A. & Derrick, K.S. Plant Mol Biol (1998) 38: 775. doi:10.1023/A:1006039016393 Abstract
A protein associated with citrus blight (CB), a disease of unknown cause, was partially characterized. The 12 kDa protein, designated p12, is diagnostic of CB and is present in leaves and xylem fluid from roots and stems of CB-affected trees. The protein, and up to six other CB-specific proteins, are readily detected by SDS-PAGE of xylem fluid from CB-affected trees. The partial N-terminal amino acid sequence of p12 was found to be unique based on database searches. A cDNA library from CB-affected root cambium was screened with a 60 bp fragment, obtained by PCR amplification of cDNA with degenerate primers designed using the amino acid sequence of p12, and two clones were selected. These clones were sequenced revealing a 674 nucleotide cDNA with a 393 nt ORF which included sequence predicted by the N-terminal amino acid sequence of p12. The amino acid sequence based on the p12 ORF was found to be up to 49% similar and 31% identical to expansins. Bacterial expression of the cloned ORF, which encodes an 11.8 kDa protein plus an N-terminal hydrophobic signal peptide, produced an immunoreactive protein of the expected size. By northern blot analysis, it was determined that p12 transcripts are present in root and stem cambium, but not in leaves of CB-affected trees, suggesting transport of the protein to leaves. Southern hybridization analysis of citrus genomic DNA indicated that p12 is a citrus encoded protein.p>
citrus blight expansin PR protein stress response References
Albrigo LG, Young RH: Phloem zinc accumulation in citrus trees affected with blight. HortScience 16: 158–160 (1981).
Allen LH Jr., Cohen M: Water stress and stomatal diffusion resistance in citrus affected with blight and young tree decline. Proc Fla State Hort Soc 87: 96–101 (1974).
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 215: 403–410 (1990).
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Current Protocols in Molecular Biology, vol 1. John Wiley, New York (1996).
Bausher MG, Sweeney MJ: Field detection of citrus blight using immunological techniques. Plant Dis 75: 447–450 (1991).
Brlansky RH: Cytology histopathology and histochemistry of citrus blight. In: Biggs AR (ed) Handbook of Cytology Histology and Histochemistry of Fruit Tree Diseases, pp. 237–251. CRC Press, New York (1993).
Brlansky RH, Lee RF, Collins MH: Structural comparison of xylem occlusions in the trunks of citrus trees with blight and other decline diseases. Phytopathology 75: 145–150 (1985).
Chomczynski P: One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal Biochem 201: 134–139 (1992).
Close TJ, Fenton RD, Moonan F: A view of plant dehydrins using antibodies specific to the carboxy terminal peptide. Plant Mol Biol 23: 279–286 (1993).
Close TJ, Fenton RD, Yang A, Asghar R, DeMason DA, Crone DE, Meyer NC, Moonan F: Dehydrin, the protein. In: Close TJ, Bray EA (eds) Plant Responses to Cellular Dehydration during Environmental Stress, pp. 104–118. American Society of Plant Physiology (1993).
Close TJ, Kortt AA, Chandler PM: A cDNA-based comparison of dehydration induced proteins (dehydrins) in barley and corn. Plant Mol Biol 13: 95–108 (1989).
Cohen M: Citrus blight and ‘blight-like’ disease. Citrus Ind 47: 12–26 (1968).
Cohen M: Diagnosis of young tree decline, blight and sand hill decline by measurement of water uptake using gravity injection. Plant Dis Rep 58: 801–805 (1974).
Cosgrove DJ: Characterization of long-term extension of isolated cell walls from growing cucumber hypocotyls. Planta 177: 121–130 (1989).
Cosgrove DJ, Bedinger P, Durachko DM: Group I allergens of grass pollen as cell wall-loosening agents. Proc Natl Acad Sci USA 94: 6559–6564 (1997).
Dellaporta, SL, Wood J, Hicks JB: A plant DNA minipreparation: Version II. Plant Mol Biol Rep 1: 9–21 (1983).
Derrick KS, Barthe GA, Hewitt BG, Lee RF: Serological tests for citrus blight. In: Moreno P, da Graca JV, Timmer LW (eds) Proceedings 12th Conference International Organization of Citrus Virologists, New Delhi, India, pp. 121–126 (1993).
Derrick KS, Barthe GA, Hewitt BG, Lee RF, Albrigo LG: Detection of citrus blight by serological assays. Proc Fla State Hort Soc 105: 26–28 (1992).
Derrick KS, Lee RF, Brlansky RH, Timmer LW, Hewitt BG, Barthe GA: Proteins associated with citrus blight. Plant Dis 74: 168–170 (1990).
Harlow E, Lane DP: Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1988).
Hecker KH, Roux KH: High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR. BioTechniques 20: 478–485 (1996).
Konat GW, Laszkiewicz I, Grubinska B, Wiggins RC: Generation of labeled DNA probes by PCR: In: Griffin HG, Griffin AM (eds) PCR Technology: Current Innovations, pp. 37–42. CRC Press, New York (1994).
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 (1970).
Lee RF, Marais LJ, Timmer LW, Graham JH: Syringe injection of water into the trunk: a rapid diagnostic test for citrus blight. Plant Dis 68: 511–513 (1984).
McCarty DR, Hattori T, Carson CB, Vasil V, Lazar M, Vasil IK: The viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell 66: 895–905 (1991).
McQueen-Mason S, Durachko DM, Cosgrove DJ: Two endogenous proteins that induce cell wall extension in plants. Plant Cell 4: 1425–1433 (1992).
Nicoletti VG, Condorelli DF: Optimized PEG method for rapid plasmid DNA purification: high yield from ‘midi-prep’. BioTechniques 14: 535–536 (1993).
Podivinsky E, Walton EF, McLeay PM: Extraction of RNA from kiwifruit tissues. BioTechniques 16: 396–398 (1994).
Prescott A, Martin C: A rapid method for the quantitative assessment of levels of specific mRNAs in plants. PlantMol Biol Rep 4: 219–224 (1987).
Rost B, Sander C: Prediction of protein structure at better than 70% accuracy. J Mol Biol 232: 584–599 (1993).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sharon A, Bailey BA, McMurty JP, Taylor R, Anderson JD: Characteristics of ethylene biosynthesis-inducing xylanase movement in tobacco leaves. Plant Physiol 100: 2059–2065 (1992).
Shcherban TY, Shi J, Durachko DM, Guiltinan MJ, McQueen-Mason SJ, Shieh M, Cosgrove DJ: Molecular cloning and sequence analysis of expansin: a highly conserved, multigene family of proteins that mediate cell wall extension in plants. Proc Natl Acad Sci USA 92: 9245–9249 (1995).
Smith PF: History of citrus blight in Florida. Citrus Ind 55: 13–18 (1974).
Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW: Use of T7 RNA polymerase to detect expression of cloned genes. Meth Enzymol 185: 60–89 (1990).
Swingle WT, Webber HJ: The principal disease of citrous fruits in Florida. Bulletin 8, pp. 9–14. USDA Division of Vegetable Physiology and Pathology (1896).
Syvertsen JP, Bausher MG, Albrigo LG: Water relations and related leaf characteristics of healthy and blight affected citrus trees. J Am Soc Hort Sci 105: 431–434 (1980).
Timmer LW, Brlansky RH, Derrick KS, Lee RF: Transmission of citrus blight by root graft inoculation. In: Brlansky RH, Lee RF, Timmer LW(eds) Proceedings 11th Conference of the International Organization of Citrus Virologists, pp. 244–249 (1991).
Tucker DPH, Lee RF, Timmer LW, Albrigo LG, Brlansky RH: Experimental transmission of citrus blight. Plant Dis 68: 979–980 (1984).
Wu Y, Sharp RE, Durachko DM, Cosgrove DJ: Growth maintenance of the maize primary root at low water potentials involves increases in cell-wall extension properties, expansin activity, and wall susceptibility. Plant Physiol 111: 765–772 (1996).
Wutscher HK, Derrick KS: Rooted air-layered cuttings from blight-affected orange trees free of blight-specific proteins. Plant Dis 77: 1264 (1993).
Wutscher HK, Cohen M, Young RH: Zinc and water-soluble phenolic levels in the wood for the diagnosis of citrus blight. Plant Dis Rep 61: 572–576 (1977).
Wutscher HK, Youtsey CO, Smith PF, cohen M: Negative results in citrus blight transmission tests. Proc Fla State Hort Soc 96: 48–50 (1983).
Google Scholar Copyright information
© Kluwer Academic Publishers 1998