Plant Molecular Biology

, Volume 38, Issue 4, pp 587–596

Water-deficit-responsive proteins in maritime pine

  • Paulo Costa
  • Nasser Bahrman
  • Jean-Marc Frigerio
  • Antoine Kremer
  • Christophe Plomion
Article

DOI: 10.1023/A:1006006132120

Cite this article as:
Costa, P., Bahrman, N., Frigerio, JM. et al. Plant Mol Biol (1998) 38: 587. doi:10.1023/A:1006006132120

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was used to identify drought-responsive proteins during progressive water deprivation of two-year old maritime pine seedlings. Stress was applied by withholding water during vegetative growth. Needles were sampled before, during and after the stress. Out of about 1000 spots that were quantified by computer analysis, 38 responded during stress. Some proteins were accumulated while others were suppressed. One to three internal microsequences were obtained for 11 proteins, 10 of which were identified on the basis of sequence homologies. These proteins are quite diverse and are involved in photosynthesis, cell elongation, antioxidant metabolism and lignification.

Pinus pinasterdroughtproteinstwo-dimensional gel electrophoresismicrosequenceneedleswater stress

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Paulo Costa
    • 1
  • Nasser Bahrman
    • 1
  • Jean-Marc Frigerio
    • 1
  • Antoine Kremer
    • 1
  • Christophe Plomion
    • 1
  1. 1.INRA, Laboratoire de Génétique et Amélioration des Arbres Forestiers, BP45CestasFrance