Plant Molecular Biology

, Volume 36, Issue 2, pp 297–306

Two maize genes encoding ω-3 fatty acid desaturase and their differential expression to temperature

  • Thomas Berberich
  • Mariko Harada
  • Kazuyuki Sugawara
  • Hiroaki Kodama
  • Koh Iba
  • Tomonobu Kusano
Article

DOI: 10.1023/A:1005993408270

Cite this article as:
Berberich, T., Harada, M., Sugawara, K. et al. Plant Mol Biol (1998) 36: 297. doi:10.1023/A:1005993408270

Abstract

We have isolated two maize cDNAs and the corresponding genes encoding fatty acid desaturase with Arabidopsis thaliana FAD7 gene as a probe. They shared almost 90% identity at DNA sequence level. Northern analysis revealed that both genes are expressed in leaves, but not in roots at normal temperature- and low temperature-growth condition. The overall level of these transcripts are elevated upon exposure to low temperature. The tissue-specific expression and DNA sequence data indicate that both genes encode plastidic ω-3 fatty acid desaturases. One of them is expressed exclusively at normal temperature but not at 5 °C , whereas the other is expressed inversely. We, therefore, termed them ZmFAD7 and ZmFAD8, respectively. Among other stresses, high-salt treatment induced the accumulation of the ZmFAD7 and ZmFAD8 transcripts in roots but drought had no effect on their expression. Cycloheximide induced the accumulation of the ZmFAD7 transcript in roots. The genomic clones of ZmFAD7 and ZmFAD8 consist of 8 exons and 7 introns as same as in the cases of A. thaliana FAD7 and FAD8 genes and the sizes of the 6 internal exons were identical among them. A phylogenetic analysis of ZmFAD7, ZmFAD8 amino acid sequences and those originated from other plant species is also presented.

gene cloningfatty acid desaturasetemperatureZea mayschloroplast

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Thomas Berberich
    • 1
  • Mariko Harada
    • 1
  • Kazuyuki Sugawara
    • 1
  • Hiroaki Kodama
    • 2
  • Koh Iba
    • 2
  • Tomonobu Kusano
    • 1
    • 4
  1. 1.Biotechnology InstituteAkita Prefectural College of AgricultureOhgata, AkitaJapan
  2. 2.Department of Biology, Faculty of ScienceKyushu UniversityFukuokaJapan
  3. 3.Botanisches Institut, J.WGoethe-UniversitätFrankfurt am MainGermany
  4. 4.Research and Education Center for Genetic InformationNora Institute of Science and TechnologyIkoma, NaraJapan