Genetic transformation of gentian using wild-type Agrobacterium rhizogenes

  • Keizo Hosokawa
  • Rikyu Matsuki
  • Yayoi Oikawa
  • Saburo Yamamura
Article

DOI: 10.1023/A:1005962913491

Cite this article as:
Hosokawa, K., Matsuki, R., Oikawa, Y. et al. Plant Cell, Tissue and Organ Culture (1997) 51: 137. doi:10.1023/A:1005962913491

Abstract

Agrobacterium rhizogenes A4 harboring the agropine type root-inducing plasmid (pRiA4) induced hairy roots on stem sections of gentian plants. Transgenic gential plants were regenerated from the hairy roots at a frequency of 19% adventitious shoot regeneration on Murashige and Skoog medium containing 30 g l-1 sucrose, 10 mg l-1 N-phenyl-N′-1,2,3-thiadiazol-5-yl urea, 1 mg l-1 1-naphthaleneacetic acid and 2 g l-1 gellan gum. The shoots were grown to maturity in a growth chamber after acclimatization. The mature transformed plants showed distinct variations in their phenotypes, such as dwarfness. Polymerase chain reaction and Southern blot analysis revealed stable integration of transferred DNA.

hairy rootsRi plasmid

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Keizo Hosokawa
    • 1
  • Rikyu Matsuki
    • 1
  • Yayoi Oikawa
    • 1
  • Saburo Yamamura
    • 1
  1. 1.Iwate Biotechnology Research CenterIwateJapan
  2. 2.National Institute of Agrobiological ResourcesIbarakiJapan