Plant Cell, Tissue and Organ Culture

, Volume 51, Issue 2, pp 137-140

Genetic transformation of gentian using wild-type Agrobacterium rhizogenes

  • Keizo HosokawaAffiliated withIwate Biotechnology Research Center
  • , Rikyu MatsukiAffiliated withIwate Biotechnology Research Center
  • , Yayoi OikawaAffiliated withIwate Biotechnology Research Center
  • , Saburo YamamuraAffiliated withIwate Biotechnology Research Center

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Agrobacterium rhizogenes A4 harboring the agropine type root-inducing plasmid (pRiA4) induced hairy roots on stem sections of gentian plants. Transgenic gential plants were regenerated from the hairy roots at a frequency of 19% adventitious shoot regeneration on Murashige and Skoog medium containing 30 g l-1 sucrose, 10 mg l-1 N-phenyl-N′-1,2,3-thiadiazol-5-yl urea, 1 mg l-1 1-naphthaleneacetic acid and 2 g l-1 gellan gum. The shoots were grown to maturity in a growth chamber after acclimatization. The mature transformed plants showed distinct variations in their phenotypes, such as dwarfness. Polymerase chain reaction and Southern blot analysis revealed stable integration of transferred DNA.

hairy roots Ri plasmid