Plant Molecular Biology

, Volume 36, Issue 2, pp 205–217

Analysis of T-DNA-mediated translational β-glucuronidase gene fusions

Authors

  • Sunee Kertbundit
    • Laboratorium Genetische VirologieVrije Universiteit Brussel
  • Rosario Linacero
    • Laboratorium Genetische VirologieVrije Universiteit Brussel
  • Pierre Rouzé
    • Laboratoire Associé de l'INRA (France), Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent
  • Ivan Galis
    • Laboratorium Genetische VirologieVrije Universiteit Brussel
  • Jiri Macas
    • Laboratorium Genetische VirologieVrije Universiteit Brussel
  • Francine Deboeck
  • Suzy Renckens
    • Laboratorium Genetische VirologieVrije Universiteit Brussel
  • Jean-Pierre Hernalsteens
    • Laboratorium Genetische VirologieVrije Universiteit Brussel
  • Henri De Greve
    • Laboratorium Genetische VirologieVrije Universiteit Brussel
Article

DOI: 10.1023/A:1005902730810

Cite this article as:
Kertbundit, S., Linacero, R., Rouzé, P. et al. Plant Mol Biol (1998) 36: 205. doi:10.1023/A:1005902730810

Abstract

Three random translational β-glucuronidase (gus) gene fusions were previously obtained in Arabidopsis thaliana, using Agrobacterium-mediated transfer of a gus coding sequence without promoter and ATG initiation site. These were analysed by IPCR amplification of the sequence upstream of gus and nucleotide sequence analysis. In one instance, the gus sequence was fused, in inverse orientation, to the nos promoter sequence of a truncated tandem T-DNA copy and translated from a spurious ATG in this sequence. In the second transgenic line, the gus gene was fused to A. thaliana DNA, 27 bp downstream an ATG. In this line, a large deletion occurred at the target site of the T-DNA. In the third line, gus is fused in frame to a plant DNA sequence after the eighth codon of an open reading frame encoding a protein of 619 amino acids. This protein has significant homology with animal and plant (receptor) serine/threonine protein kinases. The twelve subdomains essential for kinase activity are conserved. The presence of a potential signal peptide and a membrane-spanning domain suggests that it may be a receptor kinase. These data confirm that plant genes can be tagged as functional translational gene fusions.

Agrobacterium tumefaciensArabidopsis thalianagene fusioninsertion mutagenesishybrid proteinsserine/threonine receptor kinase

Copyright information

© Kluwer Academic Publishers 1998