Aldehyde dehydrogenase in tobacco pollen
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Acetaldehyde is one of the intermediate products of ethanolic fermentation, which can be reduced to ethanol by alcohol dehydrogenase (ADH). Alternatively, acetaldehyde can be oxidized to acetate by aldehyde dehydrogenase (ALDH) and subsequently converted to acetyl-CoA by acetyl-CoA synthetase (ACS). To study the expression of ALDHs in plants we isolated and characterized a cDNA coding for a putative mitochondrial ALDH (TobAldh2A) in Nicotiana tabacum/. TobALDH2A shows 54–60% identity at the amino acid level with other ALDHs and shows 76% identity with maize Rf2, a gene involved in restoration of male fertility in cms-T maize. TobAldh2A transcripts and protein were present at high levels in the male and female reproductive tissues. Expression in vegetative tissues was much lower and no induction by anaerobic incubation was observed. This suggests that TobALDH expression is not part of the anaerobic response, but may have another function. The use of specific inhibitors of ALDH and the pyruvate dehydrogenase (PDH) complex indicates that ALDH activity is important for pollen tube growth, and thus may have a function in biosynthesis or energy production.
- Brander KA, Kuhlemeier C: Apollen-specific DEAD-box protein related to translation initiation factor eIF-4A from tobacco. Plant Mol Biol 27: 637-649 (1995).
- Bucher M, Brändle R, Kuhlemeier C: Ethanolic fermentation in transgenic tobacco expressing Zymomonas mobilispyruvate decarboxylase. EMBO J 13: 2755-2763 (1994).
- Bucher M, Brander KA, Sbicego S, Mandel T, Kuhlemeier C: Aerobic fermentation in tobacco pollen. Plant Mol Biol 28: 739-750 (1995).
- Chaumont F, Bernier B, Buxant R, Williams ME, Levings III CS, Boutry M: Targeting the maize T-urf13 product into tobacco mitochondria confers methomyl sensitivity to mitochondrial respiration. Proc Natl Acad Sci USA 92: 1167-1171 (1995).
- Cui X, Wise RP, Schnable PS: The rf2nuclear restorer gene of male-sterile T-cytoplasm maize. Science 272: 1334-1336 (1996).
- Farres J, Wang X, Takahashi K, Cunningham SJ, Wang TT, Weiner H: Effects of changing glutamate 487 to lysine in rat and human liver mitochondrial aldehyde dehydrogenase. J Biol Chem 269: 13854-13860 (1994).
- Farres J, Wang TTY, Cunningham SJ, Weiner H: Investigation of the active site cysteine residue of rat liver mitochondrial aldehyde dehydrogenase by site-directed mutagenesis. Biochemistry 34: 2592-2598 (1995).
- Fleming AJ, Manzara T, Gruissem W, Kuhlemeier C: Fluorescent imaging of GUS activity and RT-PCR analysis of gene expression in the shoot apical meristem. Plant J 10: 745-754 (1996).
- Gavel Y, von Heijne G: Cleavage-site motifs in mitochondrial targeting peptides. Prot Engin 4: 33-37 (1990).
- Godbout R: High levels of aldehyde dehydrogenase transcripts in the undifferentiated chick retina. Exp Eye Res 54: 297-305 (1992).
- Hempel J, Kaiser R, Joernvall H: Mitochondrial aldehyde dehydrogenase from human liver. Eur J Biochem 153: 13-28 (1985).
- Ishitani M, Nakamura T, Han SY, Takabe T: Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid. Plant Mol Biol 27: 307-315 (1995).
- Kitson TM: Studies on the interaction between disulfiram and sheep liver cytoplasmic aldehyde dehydrogenase. Biochem J 175: 83-90 (1987).
- Koltunow AM, Truettner J, Cox KH, Wallroth M, Goldberg RB: Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2: 1201-1224 (1990).
- Levings III CS. Thoughts on cytoplasmic male sterility. Plant Cell 5: 1285-1290 (1993).
- Lindahl R: Aldehyde dehydrogenases and their role in carcinogenesis. Crit Rev Biochem Mol Biol 27: 283-335 (1992).
- MacDonald RC, Kimmerer TW: Metabolism of transpired ethanol by Eastern Cottonwood (Populus deltoidesBartr.). Plant Physiol 102: 173-179 (1993).
- Mandel T, Fleming AJ, Krähenbühl R, and Kuhlemeier C: Definition of constitutive expression in plants: the translation initiation factor eIF-4A gene as a model. Plant Mol Biol 29: 995-1004 (1995).
- McCue KF, Hanson AD: Salt-inducible betaine aldehyde dehydrogenase from sugar beet: cDNA cloning and expression. Plant Mol Biol 18: 1-11 (1992).
- Newman T, DeBruijn FJ, Green P, Keegstra K, Kende H, Mclntosh L, Ohlrogge J, Raikhel N, Somerville S, Thomashow M, Retzel E, Somerville C: Genes Galore: a summary of methods for accessing results from large-scale partial sequencing of anonymous ArabidopsiscDNA clones. Plant Physiol 106: 1241-1255 (1994).
- Owttrim GW, Mandel T, Trachsel H, Thomas AAM, Kuhlemeier C: Characterization of the tobacco eIF-4A gene family. Plant Mol Biol 26: 1747–1757 (1994).
- Pickett M, Gwynne DI, Buxton FP, Elliott R, Davies RW, Lockington RA, Scazzocchio C, Sealy-Lewis HM. Cloning and characterization of the aldAgene of Aspergillus nidulans. Gene 51: 217–226 (1987).
- Postma E, Verduyn C, Scheffers WA, Van den Dijken JP. Enzymic analysis of the Crabtree effect in glucose-limited cultures of Saccharomyces cerevisiae. Appl Environ Microbiol 55: 469-477 (1989).
- Rout UK, Weiner H: Involvement of serine 74 in the enzyme-coenzyme interaction of rat liver mitochondrial aldehyde dehydrogenase. Biochemistry 33: 8955-8961 (1994).
- Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
- Schoenbrunn-Hanebeck E, Laber B, Amrhein N: Slow-binding inhibition of the Escherichia colipyruvate dehydrogenase multienzyme complex by acetylphosphinate. Biochemistry 29. 4880-4885 (1990).
- Schrauwen JAM, de Groot PFM, van Herpen MMA, van der Lee T, Reynen VM, Weterings KAP, Wullems GJ: Stage-related expression of mRNAs during pollen development in lily and tobacco. Planta 182: 298-304 (1990).
- Shure M, Wessler S, Fedoroff N: Molecular identification and isolation of the waxylocus in maize. Cell 35: 225-233 (1983).
- Tadege M, Kuhlemeier C: Ethanolic fermention in tobacco pollen. Plant Mol Biol 35: 343-355.
- Tupy J, Rihova L: Changes and growth effect of pH in pollen tube culture. J Plant Physiol 115: 1-10 (1984).
- Wang X, Weiner H: Involvement of glutamate 268 in the active site of human liver mitochondrial (class 2) aldehyde dehydrogenase as probed by site-directed mutagenesis. Biochemistry 34: 237-243 (1995).
- Weretilnyk EA, Hanson AD: Molecular cloning of a plant betaine-aldehyde dehydrogenase, an enzyme implicated in adaptation to salinity and drought. Proc Natl Acad Sci USA 87: 2745-2749 (1990).
- Wisman E, Ramanna MS, Zabel P: Isolation of two independent allyl alcohol resistant Adh-1null mutants in tomato following selection of pollen and seeds. Plant Sci 95: 79-88 (1993).
- Zhang JZ, Laudencia-Chingcuanco DLL, Comai L, Li M, Harada JJ: Isocitrate lyase and malate synthase genes from Brassica napusL. are active in pollen. Plant Physiol 104: 857- 864 (1994).
- Zheng C-F, Weiner H: Role of the highly conserved histidine residues in rat liver mitochondrial aldehyde dehydrogenase as studied by site-directed mutagenesis. Arch Biochem Biophys 305: 460-466 (1993).
- Aldehyde dehydrogenase in tobacco pollen
Plant Molecular Biology
Volume 35, Issue 3 , pp 355-365
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- aldehyde dehydrogenase
- cytoplasmic male sterility
- glyoxylate cycle
- Industry Sectors