Plant Molecular Biology

, Volume 34, Issue 4, pp 659–668

GUS expression in Arabidopsis directed by 5′ regions of the pea metallothionein-like gene PsMT A

  • Anthony P. Fordham-Skelton
  • Catherine Lilley
  • P.E. Urwin
  • Nigel J. Robinson
Article

DOI: 10.1023/A:1005836632678

Cite this article as:
Fordham-Skelton, A.P., Lilley, C., Urwin, P. et al. Plant Mol Biol (1997) 34: 659. doi:10.1023/A:1005836632678

Abstract

Upstream sequences (including the first seven codons) of a metallothionein (MT)-like gene from pea, PsMT A, were fused to GUS and introduced into Arabidopsis. High-level GUS expression was detected in the roots of plants grown on MS medium, except in regions proximal to the root apex. There was precise delineation of the root-shoot boundary. In soil-grown plants there was low GUS expression and this was absent from the more mature regions of the roots. In the aerial tissues of soil-grown plants, GUS expression was restricted to hydathodes, stipules, expanding cotyledons and the following senescent tissues: leaves, cotyledons, petals, sepals, filaments, stigmas, nectaries and siliques. A 298 bp region was shown to be required for GUS expression in roots but not for expression in vegetative aerial tissues of plants grown on MS medium. This region contains predicted ethylene-responsive elements (EREs) but similar patterns of GUS expression were detected in etr1 seedlings. GUS expression was significantly higher in roots exposed to 500 nM copper, but this increase was small in proportion to expression in roots exposed to 50 nM copper.

metallothionein PsMT A roots reporter gene senescence trace metals 

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Anthony P. Fordham-Skelton
    • 1
  • Catherine Lilley
    • 2
  • P.E. Urwin
    • 2
  • Nigel J. Robinson
    • 1
  1. 1.Department of Biochemistry and Genetics, The Medical SchoolUniversity of NewcastleUK
  2. 2.Centre for Plant Biochemistry and BiotechnologyUniversity of LeedsUK