Plant Molecular Biology

, Volume 34, Issue 3, pp 417–426

An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-responsive sequence

Authors

  • Roland Schubert
    • GSF – National Research Center for Environment and HealthInstitute of Biochemical Plant Pathology
    • LMU München, Forstwissenschaftliche FakultätLehrbereich für Forstgenetik
  • Regina Fischer
    • Bayer AG, Landwirtschaftszentrum Monheim, Molecular TargetResearch and Biotechnology
    • Maiwald & PartnerEuropean Patent Attorneys
  • Rüdiger Hain
    • Bayer AG, Landwirtschaftszentrum Monheim, Molecular TargetResearch and Biotechnology
  • Peter H. Schreier
    • Bayer AG, Landwirtschaftszentrum Monheim, Molecular TargetResearch and Biotechnology
  • Günther Bahnweg
    • GSF – National Research Center for Environment and HealthInstitute of Biochemical Plant Pathology
  • Dieter Ernst
    • GSF – National Research Center for Environment and HealthInstitute of Biochemical Plant Pathology
  • Heinrich Sandermann Jr
    • GSF – National Research Center for Environment and HealthInstitute of Biochemical Plant Pathology
Article

DOI: 10.1023/A:1005830714852

Cite this article as:
Schubert, R., Fischer, R., Hain, R. et al. Plant Mol Biol (1997) 34: 417. doi:10.1023/A:1005830714852
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Abstract

Stilbene synthase (STS) is an enzyme involved in the biosynthesis of stilbenes, which are synthesized in various plants in response to pathogen attack, UV irradiation or exposure to ozone. We describe analysis of an ozone inducible STS transcript and its corresponding promoter (Vst1), combined with the β-glucuronidase (GUS) reporter gene. A single ozone pulse (0.1 µl/l, 10 h) resulted in 11-fold GUS expression. Histochemical localization of GUS activity revealed small spots distributed over the whole leaf. Cross-sections of leaf tissue showed that the Vst1 promoter was induced in palisade and spongy parenchyma cells and to a lesser extent in epidermal cells. Deletions at the 5′ end showed that a partial promoter sequence between position − 430 and− 280 constituted the ozone-responsive region, whereas for effective pathogen-inducibility sequences from− 280 to −140 have been shown to be necessary.

gene regulationozonepromoterresveratrol synthasestilbene synthase

Copyright information

© Kluwer Academic Publishers 1997