Photosynthesis Research

, Volume 53, Issue 2, pp 141–148

Quantitative analysis of 77K fluorescence emission spectra in Synechocystis sp. PCC 6714 and Chlamydomonas reinhardtii with variable PS I/PS II stoichiometries

  • Akio Murakami

DOI: 10.1023/A:1005818317797

Cite this article as:
Murakami, A. Photosynthesis Research (1997) 53: 141. doi:10.1023/A:1005818317797


Low-temperature (77 K) fluorescence emission spectra of intact cells of a cyanobacterium, Synechocystis sp. PCC 6714, and a green alga, Chlamydomonas reinhardtii, were quantitatively analyzed to examine differences in PS I/PS II stoichiometries. Cells cultured under different spectral conditions had various PS I/PS II molar ratios when estimated by oxidation-reduction difference absorption spectra of P700 (for PS I) and Cyt b-559 (for PS II) with thylakoid membranes. The fluorescence emission spectra under the Chl a excitation at 435 nm were resolved into several component bands using curve-fitting methods and the relative band area between PS II (F685 and F695) and PS I (F710 or F720) emissions was compared with the PS I/PS II stoichiometries of the various cell types. The results indicated that the PS I/PS II fluorescence ratios correlated closely with photosystem stoichiometries both in Synechocystis sp. PCC 6714 and in C. reinhardtii grown under different light regimes. Furthermore, the correlation between the PS I/PS II fluorescence ratios and the photosystem stoichiometries is also applicable to vascular plants.

ChlamydomonasCyt b-559P700PS I/PS II stoichiometrySynechocystis

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Akio Murakami
    • 1
  1. 1.The Center for Analytical InstrumentsNational Institute for Basic BiologyOkazaki, AichiJapan