Article

Plant Molecular Biology

, Volume 33, Issue 2, pp 343-350

First online:

Differential transcript induction of parsley pathogenesis-related proteins and of a small heat shock protein by ozone and heat shock

  • Heidrun Eckey-KaltenbachAffiliated withInstitute of Biochemical Plant Pathology, GSF - National Research Center for Environment and Health
  • , Evi KieferAffiliated withInstitute of Biochemical Plant Pathology, GSF - National Research Center for Environment and Health
  • , Erich GrosskopfAffiliated withInstitute of Biochemical Plant Pathology, GSF - National Research Center for Environment and Health
  • , Dieter ErnstAffiliated withInstitute of Biochemical Plant Pathology, GSF - National Research Center for Environment and Health
  • , Heinrich SandermannJr.Affiliated withInstitute of Biochemical Plant Pathology, GSF - National Research Center for Environment and Health

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Abstract

Parsley (Petroselinum crispum L.) is known to respond to pathogen attack by the synthesis of furanocoumarins and to UV irradiation by the synthesis of flavone glycosides whereas ozone treatment results in the induction of both pathways. A cDNA library from parsley plants was differentially screened using labelled reverse-transcribed poly(A)+ RNA isolated from ozone-treated parsley plants. This resulted in the isolation of 13 independent cDNA clones representing ozone-induced genes and of 11 cDNA clones representing ozone-repressed genes. DNA sequencing of several clones resulted in the identification of pathogenesis-related protein 1-3 (PR1-3), of a new member of PR1 cDNAs (PR1-4) and of a small heat shock protein (sHSP). Northern blot analyses showed a transient induction of the three mRNA species after ozone fumigation. In contrast, heat shock treatment of parsley plants resulted in an increase of sHSP mRNA whereas no increase for transcripts of PR1-3 and PR1-4 could be observed. This is the first characterized sHSP cDNA clone for plants induced by heat shock, as well as by oxidative stress caused by ozone.

gene expression heat shock oxidative stress ozone pathogenesis-related protein Petroselinum crispum