Plant Molecular Biology

, Volume 33, Issue 5, pp 943–946

Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment

  • Leslie van der Fits
  • Johan Memelink

DOI: 10.1023/A:1005763605355

Cite this article as:
van der Fits, L. & Memelink, J. Plant Mol Biol (1997) 33: 943. doi:10.1023/A:1005763605355


Activities of several CaMV 35S and FMV 34S promoter derivatives fused to the gusA reporter gene were compared in suspension-cultured Catharanthus roseus cells that were transiently and stably transformed using particle bombardment. Our data demonstrate that the 35S and a deletion derivative of the 34S promoter combined with particle bombardment form useful tools for genetic engineering of C. roseus cells. Our results disagree on several points with activities of 35S and 34S promoter derivatives reported for tobacco, indicating that absolute and relative promoter activities can differ between plant species.

CaMV 35S promoterCatharanthus roseusFMV 34S promoterparticle bombardmenttransformation

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Leslie van der Fits
    • 1
  • Johan Memelink
    • 1
  1. 1.Institute of Molecular Plant Sciences, Clusius LaboratoryLeiden UniversityLeidenthe Netherlands