Biogeochemistry

, Volume 36, Issue 2, pp 153–172

Investigation of the methyl fluoride technique for determining rhizospheric methane oxidation

Authors

  • JOANNE E. LOMBARDI
    • Department of ChemistryFlorida State University
  • MICHELLE A. EPP
    • Department of OceanographyFlorida State University
  • JEFFREY P. CHANTON
    • Department of OceanographyFlorida State University
Article

DOI: 10.1023/A:1005750201264

Cite this article as:
LOMBARDI, J.E., EPP, M.A. & CHANTON, J.P. Biogeochemistry (1997) 36: 153. doi:10.1023/A:1005750201264

Abstract

Methane oxidation rates in the rhizosphere of Pontederia cordata,Sagittaria lancifolia, and Typha latifolia were quantified in fieldstudies using the methyl fluoride inhibition technique. An averageoxidation of 22.9 ± 17.7% (sd,n = 44) was found for all field experiments (oxidation is expressedas a % of total potential emission in the presumedabsence of oxidation). Greenhouse experiments using the same techniquegave an average rhizospheric oxidation of 64.9 ±17.0% (sd, n = 44). Comparison of a subset ofgreenhouse plants with the methyl fluoride (MF) and a light oxic/darkanoxic (LO/DA) technique for suppressing CH4 oxidationyielded similar percentages (57.7 ±15.0% for MF and 58.5 ±13.9% for LO/DA, n = 11). Rhizospheric oxidationdisplayed a seasonal trend in Typha latifolia with decreasingoxidation percentages during warmer months as the importance ofrhizospheric CH4 oxidation declined relative toCH4 emission (46.5 ±13.8% in December and 13.5 ±1.7% in July). However, the absolute rateof methane oxidation was highest during the warmer months (44.2± 3.4 mg m-2 d-1 inDecember and 318.7 ± 151.4 mg m-2d-1 in July). As methane emission rates increased,the sensitivity of the methyl fluoride technique decreased dueto the larger error between replicate flux measurements.

aquatic marcrophytesmethanemethane oxidationmethyl fluorideplant/microbialinteractionsrhizosphere

Copyright information

© Kluwer Academic Publishers 1997