Article

Biotechnology Letters

, Volume 22, Issue 11, pp 941-945

First online:

One-step partial purification of Solanum tuberosum tuber lectin using magnetic chitosan particles

  • Mirka ŠafaříkováAffiliated withLaboratory of Biochemistry and Microbiology, Institute of Landscape Ecology
  • , Ivo ŠafaříkAffiliated withLaboratory of Biochemistry and Microbiology, Institute of Landscape Ecology

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Abstract

A one-step procedure for a partial purification of Solanum tuberosum tuber lectin has been developed. Lectin from tuber extract or from potato wastewater was adsorbed to magnetic chitosan particles and eluted with low pH buffer. The specific activity of separated lectin increased 13 times during the purification process and the recovery was 50%.

chitosan lectin magnetic particles Solanum tuberosum