Biotechnology Letters

, Volume 22, Issue 5, pp 399–405

Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities

  • Tong Zhang
  • Herbert H.P. Fang

DOI: 10.1023/A:1005680803442

Cite this article as:
Zhang, T. & Fang, H.H. Biotechnology Letters (2000) 22: 399. doi:10.1023/A:1005680803442


Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA profiles were objectively digitized using an image analyzer; the individual microbial species in a community can thus be precisely quantified. The similarity between various microbial communities was compared to the digitized DGGE profiles using the cluster analyses technique. The microbial community in a biofilm was considerably different from that in suspended sludge obtained from the same system.

biofilm cluster analysis DGGE microbial community 

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Tong Zhang
    • 1
  • Herbert H.P. Fang
    • 1
  1. 1.Centre for Environmental Engineering Research, Department of Civil EngineeringThe University of Hong KongHong Kong SAR, China

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