Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities
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- Zhang, T. & Fang, H.H. Biotechnology Letters (2000) 22: 399. doi:10.1023/A:1005680803442
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Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA profiles were objectively digitized using an image analyzer; the individual microbial species in a community can thus be precisely quantified. The similarity between various microbial communities was compared to the digitized DGGE profiles using the cluster analyses technique. The microbial community in a biofilm was considerably different from that in suspended sludge obtained from the same system.