Biotechnology Letters

, Volume 22, Issue 7, pp 561–567

On-line measurement of green fluorescent protein (GFP) fluorescence for the monitoring of recombinant adenovirus production

  • Philippe-Alexandre Gilbert
  • Alain Garnier
  • Danielle Jacob
  • Amine Kamen
Article

DOI: 10.1023/A:1005672410905

Cite this article as:
Gilbert, PA., Garnier, A., Jacob, D. et al. Biotechnology Letters (2000) 22: 561. doi:10.1023/A:1005672410905

Abstract

An on-line fluorescence sensor prototype was constructed to monitor the production of the green fluorescent protein (GFP) by 293S cells infected with a recombinant adenovirus vector (rAdV) containing the GFP gene. Fluorescence was correlated to GFP concentration and therefore to viral protein expression, but not to rAdV production. The sensor signal can also be used to compute the GFP production rate, which predicts well the occurrence of maximum viral titer.

GFPmammalian cell cultureon-line fluorescence monitoringrecombinant adenovirus production

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Philippe-Alexandre Gilbert
    • 1
  • Alain Garnier
    • 1
  • Danielle Jacob
    • 2
  • Amine Kamen
    • 2
  1. 1.Biotechnology Research Institute, Bioprocess SectorNational Research Council of CanadaMontrealCanada
  2. 2.Département de génie chimique, Faculté des sciences et de génieUniversité LavalSainte-FoyCanada