Continuous malolactic fermentation by Oenococcus Oeni entrapped in LentiKats
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
A continuous process to deacidify apple juices and cider was developed by entrapping Oenococcus oeni in LentiKats, a new polyvinyl alcohol hydrogel. For a residence time of 0.55 h, malic acid was completely converted into lactic acid when the LentiKats bioreactor was fed with apple juice at pH 4.46 and 3.95 and thirty three percent of initial malic acid (6.7 g l−1) was converted when the initial apple juice pH was 2.30. The optimal malolactic activity of this bioreactor was obtained at 30 °C and a 50% reduction in malic acid conversion was measured between 15 °C and 20 °C, at a residence time of 0.3 h. The LentiKats bioreactor gave better performance than continuous reactor with Oenococcus oeni immobilised in alginate beads (specific malic acid consumption increased by a factor of 4.6) due to the increase of the ratio external surface to volume, allowing better mass transfer.
- Cabranes, C, Moreno, J, Mangas, JJ (1998) Cider production with immobilized Leuconostoc oenos.. J. Inst. Brew. 104: pp. 127-130
- Davis, CR, Wibowo, D, Eschenbruch, R, Lee, TH, Fleet, GH (1985) Practical implications of malolactic fermentation: a review.. Am. J. Enol. Vitic 36: pp. 290-301
- Ding WA, Vorlop KD (1995) Gel aus Polyvinylalkohol und Verfahren zu seiner Herstellung. Patent DE 4327923.
- Durieux, A, Garre, V, Mukamana, J, Jourdain, JM, Silva, D, Plaisant, AM, Defroyennes, JP, Foroni, G, Simon, JP Leuconostoc oenos entrapment: applications to continuous malolactic fermentation. In: Wijffels, RH, Buitelaar, RM, Bucke, C, Tramper, J eds. (1996) Immobilized Cells: Basics and Applications. Elsevier Sciences, Amsterdam, pp. 679-686
- Jarvis B, Forster MJ, Kinsella WP (1995) Factors affecting the development of cider flavour. J. Appl. Bacteriol. Symp. Suppl. 79: 5S–18S.
- Jekel, M, Buhr, A, Wilke, T, Vorlop, KD (1998) Immobilization of biocatalysts in LentiKats.. Chem. Eng. Technol. 21: pp. 275-278
- Lonvaud-Funel, A, Strasser de Saad, AM (1982) Purification and properties of a malolactic enzyme from a strain of Leuconostoc mesenteroides isolated from grapes.. Appl. Environ. Microbiol 43: pp. 357-361
- Masschelein, CA, Ryder, DS, Simon, JP (1994) Immobilized cell technology in beer production.. Crit. Rev. Biotechnol. 14: pp. 155-177
- Nedovic, VA, Durieux, A, Van Nedervelde, L, Rosseels, P, Vandegans, J, Plaisant, AM, Simon, JP (2000) Continuous cider fermentation with co-immobilized yeast and Leuconostoc oenos cells. Enzyme Microbiol. Technol. 26: pp. 834-839
- Nielsen, JC, Richelieu, M (1999) Control of flavor development in wine during and after malolactic fermentation by Oenococcus oeni.. Appl. Environ. Microbiol. 65: pp. 740-745
- Plieva, FM, Kochetkov, KA, Singh, I, Parmar, VS, Belokon, YN, Lozinsky, VI (2000) Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for biocatalysis in water-poor media.. Biotechnol. Lett. 22: pp. 551-554
- Prüsse, U, Fox, B, Kirchkoff, M, Bruske, F, Bredford, J, Vorlop, K-D (1998) New process (jet cutting method) for the production of spherical beads from highly viscous polymer solutions.. Chem. Eng. Technol. 21: pp. 29-33
- Salou, P, Loubiere, P, Pareilleux, A (1994) Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose.. Appl. Environ. Microbiol. 60: pp. 1459-1466
- Senuma, Y, Lowe, C, Zweifel, , Hilborn, JG, Marison, I (2000) Alginate hydrogel microspheres and microcapsules prepared by spinning disk atomization.. Biotechnol. Bioeng. 67: pp. 616-622
- Spettoli, P, Nuti, MP, Zamorani, A (1984) Properties of malolactic activity purified from Leuconostoc oenos ML34 by affinity chromatography.. Appl. Environ. Microbiol. 48: pp. 900-901
- Wittlich P, Lutz J, Reimann C, Wilke T, Vorlop K-D (1999) Bioconversion of glycerol to 1,3-Propanediol. In: Proceedings of the 6th Symposium on Renewable Resources (Bonn), pp. 524–532. ISBN 3–7843–3019–3.
- Continuous malolactic fermentation by Oenococcus Oeni entrapped in LentiKats
Volume 22, Issue 21 , pp 1679-1684
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- continuous fermentation
- Oenococcus oeni
- Industry Sectors