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Monitoring growth of the methanogenic archaea Methanobacterium formicicum using an electronic nose

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Abstract

Growth of the methanogenic archaea, Methanobacterium formicicum, in pure culture was monitored by analysing samples from the gas phase with an array of chemical gas sensors (an `electronic nose'). Analyses of the methane and protein formation rates were used as independent parameters of growth, and the data obtained from the electronic nose were evaluated using principal component analysis (PCA). We found that different growth phases can be distinguished with the electronic nose followed by PCA evaluation. The fast response of the sensors in combination with the high correlations with other parameters measuring growth show that the electronic nose can be a useful tool to rapidly determine methanogenic growth.

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Authors and Affiliations

  1. Swedish Institute of Agricultural Engineering, P.O. Box 7033, S-750 07, Uppsala, Sweden

    Jennie Brandgård & Åke Nordberg

  2. Department of Microbiology, SLU, P.O. Box 7025, S-750 07, Uppsala, Sweden

    Ingvar Sundh, Anna Schnürer & Berit Mathisen

  3. Division of Biotechnology and Swedish Sensor Centre, Department of Physics and Measurement Technology, Linköping University, S-581 83, Linköping, Sweden

    Carl-Fredrik Mandenius

Authors
  1. Jennie Brandgård
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  2. Ingvar Sundh
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  3. Åke Nordberg
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  4. Anna Schnürer
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  5. Carl-Fredrik Mandenius
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  6. Berit Mathisen
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Brandgård, J., Sundh, I., Nordberg, Å. et al. Monitoring growth of the methanogenic archaea Methanobacterium formicicum using an electronic nose. Biotechnology Letters 23, 241–248 (2001). https://doi.org/10.1023/A:1005643606640

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