Biotechnology Letters

, Volume 21, Issue 11, pp 997–1002

Genetic transformation via particle bombardment of Catharanthus roseus plants through adventitious organogenesis of buds

Authors

  • Rafael Zárate
    • Division of Pharmacognosy, Gorlaeus laboratories, Leiden/Amsterdam Center for Drug Research (LACDR)Leiden University
  • Johan Memelink
    • Institute of Molecular Plant Sciences, Clusius LaboratoryLeiden University
  • Robert van der Heijden
    • Division of Pharmacognosy, Gorlaeus laboratories, Leiden/Amsterdam Center for Drug Research (LACDR)Leiden University
  • Robert Verpoorte
    • Division of Pharmacognosy, Gorlaeus laboratories, Leiden/Amsterdam Center for Drug Research (LACDR)Leiden University
Article

DOI: 10.1023/A:1005622317333

Cite this article as:
Zárate, R., Memelink, J., van der Heijden, R. et al. Biotechnology Letters (1999) 21: 997. doi:10.1023/A:1005622317333

Abstract

In vitro propagation of Catharanthus roseus was achieved using nodal explants. Bud induction was best on medium containing 1.0 mg benzyl aminopurine l−1. Hardening of rooted shoots to soil was very successful with 98% survival. Genetically transformed C. roseus plantlets were obtained after bombardment of nodal explants, which were then micropropagated, with DNA coated particles with green fluorescent protein (GFP) or β-glucuronidase (GUS) reporter genes. Histological studies showed that the gene insertion method proved effective with many cells and different tissues displaying the reporter gene signals, showing that gene expressions were rather stable.

β-glucuronidaseCatharanthus roseusgenetic transformationgreen fluorescent proteinparticle bombardment

Copyright information

© Kluwer Academic Publishers 1999