Biotechnology Letters

, Volume 21, Issue 11, pp 925–927

Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive

  • Dong-Hyun Kim
  • Byung-Chul Oh
  • Won-Chan Choi
  • Jung-Kee Lee
  • Tae-Kwang Oh
Article

DOI: 10.1023/A:1005602717835

Cite this article as:
Kim, DH., Oh, BC., Choi, WC. et al. Biotechnology Letters (1999) 21: 925. doi:10.1023/A:1005602717835

Abstract

Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosphate while 3 mM phosphate inhibited 50% of the original activity in case of A. ficuum phytase. Addition of 5 mM CaCl2 significantly broadened the active pH range and also increased the pH stability of DS11 phytase.

calcium pH stability phosphate inhibition phytase thermal stability 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Dong-Hyun Kim
    • 1
  • Byung-Chul Oh
    • 1
  • Won-Chan Choi
    • 1
  • Jung-Kee Lee
    • 1
  • Tae-Kwang Oh
    • 1
  1. 1.Korea Research Institute of Bioscience and BiotechnologyYusong, TaejonKorea