Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive
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- Kim, DH., Oh, BC., Choi, WC. et al. Biotechnology Letters (1999) 21: 925. doi:10.1023/A:1005602717835
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Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosphate while 3 mM phosphate inhibited 50% of the original activity in case of A. ficuum phytase. Addition of 5 mM CaCl2 significantly broadened the active pH range and also increased the pH stability of DS11 phytase.